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  • KD/KO Validated

IRAK1 Polyclonal antibody

IRAK1 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10478-2-AP


IRAK, IRAK 1, IRAK1, pelle

Tested Applications

Positive WB detected inA549 cells, PC-13 cells, PC-12 cells, RAW 264.7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10478-2-AP targets IRAK1 in WB, RIP, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK1 fusion protein Ag0728
Full Name interleukin-1 receptor-associated kinase 1
Calculated molecular weight 77 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC014963
Gene symbol IRAK1
Gene ID (NCBI) 3654
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)


Product Specific Protocols
WB protocol for IRAK1 antibody 10478-2-APDownload protocol
IHC protocol for IRAK1 antibody 10478-2-APDownload protocol
IF protocol for IRAK1 antibody 10478-2-APDownload protocol
IP protocol for IRAK1 antibody 10478-2-APDownload protocol
FC protocol for IRAK1 antibody 10478-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Targeting of microRNA-142-3p in dendritic cells regulates endotoxin-induced mortality.

Authors - Sun Yaping Y

J Crohns Colitis

A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages.

Authors - Feihong Deng

Cell Mol Immunol

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Authors - Floriana Mulas

Cell Mol Immunol

Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation.

Authors - Gang Xu
humanWB, IHC


S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.

Authors - Dong-Fang Meng

Int J Radiat Oncol Biol Phys

miR-142a-3p enhances FlaA N/C protection against radiation-mediated intestinal injury by modulating IRAK1/NF-κB signaling pathway

Authors - Teng Liu
  • KD Validated