IRAK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IRAK1 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 10478-2-AP

Synonyms

IRAK, IRAK 1, IRAK1, pelle

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 10478-2-AP

  WB result of IRAK1 antibody (10478-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells.

• 

  WB analysis of A549 using 10478-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 10478-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10478-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10478-2-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10478-2-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10478-2-AP (IRAK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10478-2-AP

  IP Result of anti-IRAK1 (IP:10478-2-AP, 4ug; Detection:10478-2-AP 1:600) with HeLa cells lysate 2800ug.

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10478-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10478-2-AP (IRAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10478-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRAK1 antibody (10478-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).

• 

  FC experiment of HeLa using 10478-2-AP

  1X10^6 HeLa cells were stained with 0.20ug IRAK1 antibody (10478-2-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A549 cells, PC-13 cells, PC-12 cells, RAW 264.7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

10478-2-AP targets IRAK1 in WB, RIP, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRAK1 fusion protein Ag0728
• Full Name: interleukin-1 receptor-associated kinase 1
• Calculated molecular weight: 77 kDa
• Observed molecular weight: 68-80 kDa
• GenBank accession number: BC014963
• Gene symbol: IRAK1
• Gene ID (NCBI): 3654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

Publications

Species	Application	Title
human	WB	Blood; Targeting of microRNA-142-3p in dendritic cells regulates endotoxin-induced mortality.; Authors - Sun Yaping Y
mouse	WB,IHC	J Crohns Colitis; A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages.; Authors - Feihong Deng
mouse	WB	Cell Mol Immunol; The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.; Authors - Floriana Mulas
human	WB	Cell Mol Immunol; Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation.; Authors - Gang Xu
human	WB, IHC	Oncogene; S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.; Authors - Dong-Fang Meng
mouse	WB,IF	Int J Radiat Oncol Biol Phys; miR-142a-3p enhances FlaA N/C protection against radiation-mediated intestinal injury by modulating IRAK1/NF-κB signaling pathway; Authors - Teng Liu ; KD Validated

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