- Featured Product
- KD/KO Validated
IRAK1 Polyclonal antibody
IRAK1 Polyclonal Antibody for FC (Intra), IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, RIP, IP, IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 10478-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, PC-13 cells, PC-12 cells, RAW 264.7 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 25 publications below |
IHC | See 5 publications below |
IF | See 1 publications below |
RIP | See 1 publications below |
Product Information
10478-2-AP targets IRAK1 in WB, RIP, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IRAK1 fusion protein Ag0728 |
Full Name | interleukin-1 receptor-associated kinase 1 |
Calculated molecular weight | 77 kDa |
Observed molecular weight | 68-80 kDa |
GenBank accession number | BC014963 |
Gene symbol | IRAK1 |
Gene ID (NCBI) | 3654 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)
Protocols
Product Specific Protocols | |
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WB protocol for IRAK1 antibody 10478-2-AP | Download protocol |
IHC protocol for IRAK1 antibody 10478-2-AP | Download protocol |
IF protocol for IRAK1 antibody 10478-2-AP | Download protocol |
IP protocol for IRAK1 antibody 10478-2-AP | Download protocol |
FC protocol for IRAK1 antibody 10478-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Blood Targeting of microRNA-142-3p in dendritic cells regulates endotoxin-induced mortality. | ||
J Crohns Colitis A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages. | ||
Cell Mol Immunol The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13. | ||
Cell Mol Immunol Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation. | ||
Oncogene S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1. | ||
Int J Radiat Oncol Biol Phys miR-142a-3p enhances FlaA N/C protection against radiation-mediated intestinal injury by modulating IRAK1/NF-κB signaling pathway
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