ISM1 Polyclonal antibody

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  WB analysis using 30152-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30152-1-AP (ISM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ISM1 Polyclonal Antibody for WB, ELISA

Cat No. 30152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

bA149I18.1, C20orf82, dJ1077I2.1, ISM, ISM1, Isthmin, Isthmin 1, isthmin 1 homolog (zebrafish)

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Tested Applications

• Positive WB detected in: HT-29 cells, TT cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30152-1-AP targets ISM1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISM1 fusion protein Ag32761
• Full Name: isthmin 1 homolog (zebrafish)
• Calculated Molecular Weight: 52kd
• Observed Molecular Weight: 52 kDa, 70 kDa
• GenBank Accession Number: NM_080826
• Gene Symbol: ISM1
• Gene ID (NCBI): 140862
• RRID: AB_3669703
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: B1AKI9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISM1, also known as C20orf82. It is expected to be located in extracellular space, and the protein is enriched in the thyroid gland tissue. Acts as an angiogenesis inhibitor. The protein is predicted to be involved in negative regulation of angiogenesis. Since the molecular weight of the detected ISM1 was larger than expected (52 kD), ISM1 is likely subject to posttranslational modifications. Glycosylation is commonly found in many secreted proteins, and analysis of the ISM1 protein sequence predicted two putative N-glycosylation sites at positions N39 and N282. (PMID: 31171630)