Tested Applications
| Positive WB detected in | PC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66070-1-Ig targets Integrin Alpha 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse samples.
| Tested Reactivity | human, pig, mouse |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17293 Product name: Recombinant human Integrin alpha-3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 52-376 aa of BC150190 Sequence: FGYSVALHRQTERQQRYLLLAGAPRELAVPDGYTNRTGAVYLCPLTAHKDDCERMNITVKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAHRYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDDWQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNTVYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDPEDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHRHMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAIYVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI Predict reactive species |
| Full Name | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| Calculated Molecular Weight | 1066 aa, 119 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC150190 |
| Gene Symbol | Integrin alpha 3 |
| Gene ID (NCBI) | 3675 |
| RRID | AB_11042765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| WB protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Integrin α3 (ITGA3) antibody, catalog number 66070-1-Ig, has 37 total citations published in journals including Nature, Cell, Nature Cell Biology, Nature Communications, PNAS, Cell Reports, iScience, Development, FASEB Journal, and others. Research fields span oncology (liver, pancreatic, gastric, colon, breast, glioblastoma, cervical, oral, and head/neck cancers), wound healing, fibrosis, kidney disease, cardiovascular biology, developmental biology, neuroscience, and immunology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis | ||
Nat Commun Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents. | ||
Adv Sci (Weinh) Biodegradable Cardiac Occluder with Surface Modification by Gelatin-Peptide Conjugate to Promote Endogenous Tissue Regeneration | ||
Carbohydr Polym High molecular weight laminarin/AgNPs-impregnated PVA based in situ hydrogels accelerated diabetic wound healing. |
Reviews
What customers say
Users report positive experiences with this antibody. One reviewer (5/5) used it for Western Blot on MDA-MB-231 cells at a 1:1000 overnight dilution at 4°C, noting clean bands at the correct molecular weight. Another reviewer (4/5) tested it using EDTA plasma on an antibody microarray across two lots, finding it reliable for that application. Overall, the antibody is well-received for both Western Blot and microarray-based assays, with users highlighting specificity and consistent performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ewan (Verified Customer) (07-11-2024) | Works well by western clean band and correct size
|
Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
|
Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
|
