CoraLite® Plus 488-conjugated Integrin Alpha 3 Monoclonal antibody

• Featured Product
• KD/KO Validated

Integrin Alpha 3 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F9G4

Cat no : CL488-66070

Synonyms

CD49C, FLJ34631, FLJ34704, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Validation Data Gallery

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  WB analysis using CL488-66070

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66070 (Integrin Alpha 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66070 targets Integrin Alpha 3 in WB applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Calculated Molecular Weight: 1066 aa, 119 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: BC150190
• Gene Symbol: ITGA3
• Gene ID (NCBI): 3675
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.