ITPR1-specific Polyclonal antibody

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• ITPR1-specific Polyclonal antibody (19962-1-AP)

ITPR1-specific Polyclonal Antibody for WB, IHC, IF-Fro, FC (Intra), IP, ELISA

Cat No. 19962-1-AP Copied!

Publications (49)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA

Synonyms

ITPR1, ACV, Inositol 1,4,5 trisphosphate receptor, Inositol 1,4,5-trisphosphate receptor type 1, INSP3R1

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  WB analysis of mouse brain using 19962-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19962-1-AP

  Various lysates were subjected to Tris-Acetate gel system followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 19962-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19962-1-AP (ITPR1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 19962-1-AP

  IP result of anti-ITPR1-specific (IP:19962-1-AP, 4ug; Detection:19962-1-AP 1:300) with mouse brain tissue lysate 3160 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 19962-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19962-1-AP (ITPR1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 19962-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ITPR1-specific antibody (19962-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 19962-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ITPR1-specific antibody (19962-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 19962-1-AP

  1X10^6 HepG2 cells were stained with 0.2 ug ITPR1-specific antibody (19962-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, mouse liver tissue, L02 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19962-1-AP targets ITPR1-specific in WB, IHC, IF-Fro, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine, sheep, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inositol 1,4,5-triphosphate receptor, type 1
• Calculated Molecular Weight: 314 kDa
• Observed Molecular Weight: 290-300 kDa
• GenBank Accession Number: NM_001099952
• Gene Symbol: ITPR1
• Gene ID (NCBI): 3708
• RRID: AB_10638786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Publications

What published studies show

The ITPR1-specific Polyclonal antibody (Cat# 19962-1-AP) has 49 citations across journals including Nature Communications, Neuron, Cell Discovery, EMBO Journal, and Molecular Psychiatry. Research fields span calcium signaling, mitochondria-associated membrane (MAM) biology, neurodegeneration, diabetic nephropathy, cancer, cardiovascular disease, and inflammation.

Automatically generated from the 49 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Commun; ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome; Authors - Mailis Liiv
		Nat Commun; OrthoID: profiling dynamic proteomes through time and space using mutually orthogonal chemical tools; Authors - Ara Lee
mouse	IF	Cell Discov; Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction.; Authors - Yi-yang Zhao
human		Neuron; Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.; Authors - Colleen A Stoyas
human	WB	J Exp Clin Cancer Res; ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.; Authors - Mengzhao Zhang
human	WB	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun