ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 19962-1-AP

Synonyms

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor



Tested Applications

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19962-1-AP targets ITPR1-specific in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name inositol 1,4,5-triphosphate receptor, type 1
Calculated molecular weight 314 kDa
Observed molecular weight 290-300 kDa
GenBank accession numberNM_001099952
Gene symbol ITPR1
Gene ID (NCBI) 3708
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.

Protocols

Product Specific Protocols
WB protocol for ITPR1-specific antibody 19962-1-APDownload protocol
IHC protocol for ITPR1-specific antibody 19962-1-APDownload protocol
IP protocol for ITPR1-specific antibody 19962-1-APDownload protocol
FC protocol for ITPR1-specific antibody 19962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EBioMedicine

DsbA-L ameliorates high glucose induced tubular damage through maintaining MAM integrity.

Authors - Ming Yang
humanWB

J Mol Neurosci

PMID: 28730337

Authors - Xiaolong Wang
humanWB

Proteomics Clin Appl

Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates Reveals the Mechanism of Tween-80-Induced Anaphylactoid Reaction.

Authors - Yan-Ni Mi
mouseWB

Toxicology

PMID: 29800586

Authors - Xiaolong Wang
humanIHC

Front Endocrinol (Lausanne)

MAMs Protect Against Ectopic Fat Deposition and Lipid-Related Kidney Damage in DN Patients.

Authors - Ming Yang
human

Neuron

Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7.

Authors - Colleen A Stoyas