CoraLite® Plus 647-conjugated ITPR1-specific Polyclonal antibody

ITPR1-specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-19962

Synonyms

INSP3R1, IP3 receptor isoform 1, IP3R, IP3R 1, IP3R1, ITPR1, SCA15, SCA16, Type 1 InsP3 receptor

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Validation Data Gallery

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  FC experiment of HepG2 using CL647-19962

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ITPR1-specific (CL647-19962) (red), or 0.2 ug CoraLite® Plus 647-conjugated Rabbit IgG Isotype Control (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-19962 targets ITPR1-specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inositol 1,4,5-triphosphate receptor, type 1
• Calculated Molecular Weight: 314 kDa
• Observed Molecular Weight: 290-300 kDa
• GenBank Accession Number: NM_001099952
• Gene Symbol: ITPR1
• Gene ID (NCBI): 3708
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.