CD61 / Integrin Beta 3 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD61 / Integrin Beta 3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

3D8E5

Cat no : 66952-1-Ig

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

---

Validation Data Gallery

• 

  WB analysis of HUVEC using 66952-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66952-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66952-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66952-1-Ig (CD61 / Integrin Beta 3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HUVEC cells, human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

66952-1-Ig targets CD61 / Integrin Beta 3 in WB, IP, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD61 / Integrin Beta 3 fusion protein Ag27834
• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Calculated Molecular Weight: 788 aa, 87 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC127666
• Gene Symbol: ITGB3
• Gene ID (NCBI): 3690
• RRID: AB_2882275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC	Sci Rep; Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer.; Authors - Hiroyuki Yamato ; KD Validated
human	WB	Reprod Biol Endocrinol; Expression of Talin-1 in endometriosis and its possible role in pathogenesis.; Authors - Xian Tang
human	WB,IP	BMC Biotechnol; αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.; Authors - Junxin Mai
human	WB	Acta Pharmacol Sin; Adiponectin receptor agonist AdipoRon modulates human and mouse platelet function.; Authors - Xiang-Hui Zhou
mouse	WB	Drug Deliv; Suppression for lung metastasis by depletion of collagen I and lysyl oxidase via losartan assisted with paclitaxel-loaded pH-sensitive liposomes in breast cancer; Authors - Li Zhang
human	WB,IP	Biomedicines; ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis; Authors - Liansuo Zhang