|Positive WB detected in||HUVEC cells, human placenta tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
66952-1-Ig targets Integrin Beta 3 in WB,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Integrin Beta 3 fusion protein Ag27834|
|Full Name||integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)|
|Calculated molecular weight||788 aa, 87 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC127666|
|Gene ID (NCBI)||3690|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.