Integrin Beta 3 Monoclonal antibody

Integrin Beta 3 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3D8E5

Cat no : 66952-1-Ig

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3



Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66952-1-Ig targets Integrin Beta 3 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Integrin Beta 3 fusion protein Ag27834
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC127666
Gene symbol ITGB3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 3 antibody 66952-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Reprod Biol Endocrinol

Expression of Talin-1 in endometriosis and its possible role in pathogenesis.

Authors - Xian Tang