JAM-A/CD321 Polyclonal antibody

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  WB analysis using 32747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32747-1-AP (JAM-A/CD321 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

JAM-A/CD321 Polyclonal Antibody for WB, ELISA

Cat No. 32747-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CD321, F11r, Jam1, JAM-1, JAM-A

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32747-1-AP targets JAM-A/CD321 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JAM-A/CD321 fusion protein Eg3117
• Full Name: F11 receptor
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: NM_172647.2
• Gene Symbol: F11r
• Gene ID (NCBI): 16456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O88792
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F11R, also known as Junctional Adhesion Molecule-A (JAM-A), is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily located in the tight junctions of epithelial and endothelial cells and is also expressed on circulating platelets and leukocytes (PMID: 34533648). Targeting F11R/JAM-A with monoclonal antibodies or peptide antagonists has shown promise in inhibiting tumor growth and metastasis in mouse models (PMID: 37563645).