JUN Monoclonal antibody

JUN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

24

CloneNo.

1G6B11

Cat no : 66313-1-Ig

Synonyms

Activator protein 1, AP 1, AP1, c Jun, JUN, jun oncogene, P39, Proto oncogene c Jun, Transcription factor AP 1

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Validation Data Gallery

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  WB analysis using 66313-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66313-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66313-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Saos-2 using 66313-1-Ig

  Saos-2 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66313-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66313-1-Ig (JUN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, ROS1728 cells, Saos-2 cells, HEK-293 cells, NIH/3T3 cells, RKO cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below

Product Information

66313-1-Ig targets JUN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: JUN fusion protein Ag17639
• Full Name: jun oncogene
• Calculated Molecular Weight: 331 aa, 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC068522
• Gene Symbol: JUN
• Gene ID (NCBI): 3725
• RRID: AB_2881694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of JUN?

The molecular weight of JUN is approximately 39 kDa.

What is the cellular localization of JUN?

JUN expression can be located in the nucleus. However, ubiquitinated JUN colocalizes with lysosomal proteins (PMID: 15469925).

 What is the function of JUN?

JUN is a basic leucine zipper (bZIP) transcription factor that recognizes and binds to a consensus heptamer motif of 5'-TGA[CG]TCA-3' in the enhancer region of target genes. JUN acts as either a homo- or heterodimer and binds to the DNA to regulate transcriptional activity, specifically promoting the activity of NR5A1 when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon the stimulation of the cAMP signaling pathway (PMID: 9732876).

 What post-translational modifications is JUN subjected to?

JUN is acetylated at Lys-271 by EP300 (PMID: 11689449). JUN is phosphorylated by PLK3 following exposure to hypoxia or UV irradiation, which leads to increased DNA-binding activity (PMID: 27281822), and also by PAK2 at numerous threonine residues to promote cell proliferation and transformation (PMID: 21177766). JUN is also phosphorylated by DYRK2 at Ser-243, which primes it for subsequent further phosphorylation by GSK3B and reduces its ability to bind DNA (PMID: 22307329).

 What is the role of JUN in disease?

Activation of JUN is linked with proliferation and angiogenesis in invasive breast cancers (PMID: 16733206).

Publications

Species	Application	Title
mouse	WB	J Hematol Oncol; PMID: 32552902; Authors - Xinhua Xiao
mouse,human	WB	Cell Biol Toxicol; microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.; Authors - Qiuyue Li
human	WB	Genomics; Integrative epigenomic profiling reveal AP-1 is a key regulator in intrahepatich cholangiocarcinoma.; Authors - Ke He
human	WB	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
mouse	WB	J Cell Physiol; Lack of Mof reduces acute liver injury by enhancing transcriptional activation of Igf1.; Authors - Meng Wang
mouse	WB	Front Pharmacol; Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2.; Authors - Xiang Tong