Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98243-1-RR

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human Jagged1 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98243-1-RR

  1x10^6 HEK-293T cells (left) or Jagged1/CD339 transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody (98243-1-RR, Clone:241412A6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98243-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98243-1-RR against Human Jagged1/CD339 were performed. The affinity constant is 1.57 nM.

Jagged1/CD339 Recombinant Antibody for FC

Cat No. 98243-1-RR

Clone No.241412A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Jagged1, JAG1, Protein jagged-1, HJ1, CD339

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98243-1-RR targets Jagged1/CD339 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: jagged 1 (Alagille syndrome)
• Calculated Molecular Weight: 1218 aa, 134 kDa
• GenBank Accession Number: BC098393
• Gene Symbol: Jagged1
• Gene ID (NCBI): 182
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78504
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Jagged1 (JAG1), also known as CD339, is a type I, membrane-anchored, multi-domain protein and a ligand of the Notch receptor (PMID: 15358557). Jagged1 is composed of a relatively small intracellular domain, a transmembrane domain, and a larger extracellular component containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and a signal peptide (PMID: 15358557; 26548814). It is widely expressed throughout mammalian development, across many tissues and developmental stages (PMID: 26548814). The Jagged1-NOTCH interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis.