KCNQ5 Polyclonal antibody

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  WB analysis using 28000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28000-1-AP (KCNQ5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 28000-1-AP

  IP result of anti-KCNQ5 (IP:28000-1-AP, 4ug; Detection:28000-1-AP 1:500) with Raji cells lysate 1600 ug.

KCNQ5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 28000-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Voltage-gated potassium channel subunit Kv7.5, Potassium voltage-gated channel subfamily KQT member 5, Kv7.5, KQT-like 5, KQT like 5

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Tested Applications

• Positive WB detected in: Calu-1 cells, HEL cells, Raji cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28000-1-AP targets KCNQ5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCNQ5 fusion protein Ag27622
• Full Name: potassium voltage-gated channel, KQT-like subfamily, member 5
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001160130
• Gene Symbol: KCNQ5
• Gene ID (NCBI): 56479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NR82
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Potassium voltage-gated channel subfamily Q member 5 (KCNQ5, also known as Kv7.5 and MRD46) is widely expressed in the brain and generates M-type current. It has a role in the regulation of neuronal excitability (PMID: 10816588). KCNQ5 mutations are associated with increased excitability and decreased repolarization reserve, lead to pathophysiology (PMID: 28669405).