Ki-67 Polyclonal antibody

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  IHC staining of human breast cancer using 19972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19972-1-AP (KI67 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 19972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19972-1-AP (KI67 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

Ki67 antibodies are widely used as markers for cell proliferation, enabling the detection and quantification of actively dividing cells in tissue samples for research and diagnostic applications, especially in cancer pathology. Proteintech has an extensive range of Ki-67 antibodies validated for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment and more.

Cat No. 19972-1-AP

Publications(47)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Synonyms

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 37 publications below
• IF: See 1 publications below

Product Information

19972-1-AP targets KI67 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: antigen identified by monoclonal antibody Ki-67
• Calculated Molecular Weight: 359 kDa
• GenBank Accession Number: NM_002417
• Gene Symbol: KI67
• Gene ID (NCBI): 4288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46013
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKI67 gene encodes a nuclear protein, antigen KI-67, which was identified by monoclonal antibody Ki-67. Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (PMID: 8227122). The antigen Ki-67 is a human nuclear protein the expression of which is strictly associated with cell proliferation and which is widely used in routine pathology as a "proliferation marker" to measure the growth fraction of cells in human tumors (PMID: 8227122).

Publications

Species	Application	Title
mouse	IHC	Phytomedicine; Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4; Authors - Guoqing Zhang
human	WB,IHC	J Transl Med; Molecule interacting with CasL-2 enhances tumor progression and alters radiosensitivity in cervical cancer; Authors - Yun Teng
human	IHC	J Invest Dermatol; FUT8 remodeling of EGFR regulates epidermal keratinocyte proliferation during psoriasis development.; Authors - Musin Kelel
human	IHC	Oncotarget; miR-16 targets fibroblast growth factor 2 to inhibit NPC cell proliferation and invasion via PI3K/AKT and MAPK signaling pathways.; Authors - Qingmei He
mouse	IHC	Int J Cancer; Inhibition of tumor growth by U0126 is associated with induction of interferon-γ production.; Authors - Xingzhe Ma
human	IHC	Acta Biomater; Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance.; Authors - Jiulong Zhang