KIF17 Recombinant monoclonal antibody

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  WB analysis using 85046-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85046-1-RR (KIF17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85046-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85046-1-RR (KIF17 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 85046-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KIF17 antibody (85046-1-RR, Clone: 242469G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85046-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85046-1-RR against Human KIF17 were performed. The affinity constant is below 1 pM.

KIF17 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85046-1-RR

Clone No.242469G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

242469G4, KIAA1405, KIF3-related motor protein, KIF3X, Kinesin-like protein KIF17

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85046-1-RR targets KIF17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6217

  Product name: Recombinant human KIF17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC065927

  Sequence: MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYLEIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRSHSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDGRCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALLR

• Full Name: kinesin family member 17
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC065927
• Gene Symbol: KIF17
• Gene ID (NCBI): 57576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9P2E2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).