KIF5C Polyclonal antibody, PBS Only

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  WB analysis using 25897-1-AP (same clone as 25897-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25897-1-AP (KIF5C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

  IP result of anti-KIF5C (IP:25897-1-AP, 4ug; Detection:25897-1-AP 1:800) with mouse brain tissue lysate 2080 ug. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 25897-1-AP (same clone as 25897-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF5C antibody (25897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25897-1-AP (same clone as 25897-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIF5C antibody (25897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

KIF5C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 25897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531

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Product Information

25897-1-PBS targets KIF5C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF5C fusion protein Ag23163
• Full Name: kinesin family member 5C
• Calculated Molecular Weight: 725 aa, 83 kDa
• Observed Molecular Weight: 70 kDa, 110 kDa
• GenBank Accession Number: BC110287
• Gene Symbol: KIF5C
• Gene ID (NCBI): 3800
• RRID: AB_2880288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60282
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.