KIM-1/HAVCR1 Recombinant monoclonal antibody

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  WB analysis using 83221-2-RR

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 83221-2-RR (HAVCR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83221-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83221-2-RR against Rat HAVCR1 were performed. The affinity constant is 0.477 nM.

KIM-1/HAVCR1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83221-2-RR

Clone No.230528C5

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

HAVCR1, KIM-1, TIM-1, Kim1, KIM 1

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

83221-2-RR targets KIM-1/HAVCR1 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: hepatitis A virus cellular receptor 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_173149.2
• Gene Symbol: Havcr1
• Gene ID (NCBI): 286934
• RRID: AB_3670906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O54947
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1), CD365, or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV) (PMID: 9658108; 8861957). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance (PMID: 16284246; 15793575). KIM-1 may be involved in the regulation of asthma and allergic diseases (PMID: 14534576). It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury (PMID: 14600030).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Adaptive Antioxidant Nanomedicines Inhibit Ferroptosis in Renal Tubular Epithelial Cells to Alleviate Diabetic Kidney Disease; Authors - Zerun Liu
mouse	IHC	J Nanobiotechnology; Citrate-coated Prussian blue nanozyme hitchhikes neutrophils to ameliorate calcium oxalate crystal-induced kidney injury via inhibiting pyroptosis and NETosis.; Authors - Xiaozhuo Ba
human, mouse	WB,IF	Int Immunopharmacol; KLF6 enhances ferroptosis in S-AKI through the NCOA4/ACSL4/LPCAT3 axis.; Authors - You Zhou
	WB	Life Sci; Integrated multi-omics profiling identifies Cmpk1 as a monocyte-specific therapeutic target for renal ischemia-reperfusion injury; Authors - Haoxun Zhang
mouse	WB	J Ethnopharmacol; Trishizukaol A alleviates chronic kidney disease by restraining P2X7/PSME3-driven 20S proteasome hyperactivation.; Authors - Pengfei Tang
mouse	WB	Inflammation; Salidroside Alleviates Renal Ischemia-reperfusion Injury by Inhibiting Macrophage Pyroptosis Through HIF Signaling.; Authors - Zhan Xueqiang