Tested Applications
| Positive WB detected in | Mo7e cells, HAP1 cells |
| Positive IHC detected in | stromal tumor tissue, human placenta tissue, human testis tissue, Insulinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18696-1-AP targets CD117/c-Kit in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog |
| Calculated Molecular Weight | 110 kDa |
| Observed Molecular Weight | 140-160 kDa |
| GenBank Accession Number | BC071593 |
| Gene Symbol | CD117 |
| Gene ID (NCBI) | 3815 |
| RRID | AB_2249558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10721 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD117/c-Kit antibody 18696-1-AP | Download protocol |
| IHC protocol for CD117/c-Kit antibody 18696-1-AP | Download protocol |
| WB protocol for CD117/c-Kit antibody 18696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD117/c-Kit Polyclonal antibody (Cat# 18696-1-AP) has 61 citations across journals including Science Advances, Protein & Cell, Journal of Experimental & Clinical Cancer Research, Free Radical Biology and Medicine, Molecular Medicine, Diabetes, Nature Precision Oncology, and eLife. Research fields span hematopoietic stem cell biology, gastrointestinal stromal tumors, constipation and gut motility, myocardial tissue engineering, ovarian cancer, melanocyte biology, prostate disease, oocyte development, and neurogenic bladder.
| Species | Application | Title |
|---|---|---|
Bioact Mater A biodegradable, microstructured, electroconductive and nano-integrated drug eluting patch (MENDEP) for myocardial tissue engineering | ||
Protein Cell p21/Zbtb18 repress the expression of cKit to regulate the self-renewal of hematopoietic stem cells | ||
J Exp Clin Cancer Res Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells. | ||
Sci Adv Nat10-mediated ac4C epitranscriptomics orchestrates hematopoietic stem/progenitor cell fate determination via translation control. | ||
Adv Healthc Mater ROS-Responsive Hydrogel Delivering METRNL Enhances Bone Regeneration via Dual Stem Cell Homing and Vasculogenesis Activation |
Reviews
What customers say
One reviewer (Reyes Castano Martin, Feb 2025) rated this product 4/5 stars after using it for immunofluorescence on human brain cortex (epileptic TSC FFPE tissue) at a 1:100 dilution. They observed that CD117, typically a stem cell marker, unexpectedly appeared to label some neurons in their sample, suggesting potential off-target or broader reactivity in this context. Overall, the antibody performed adequately for IF but the reviewer flagged an interesting cross-reactivity observation worth noting for interpretation.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (02-14-2025) | Interestingly, CD117 (in green), marker of stem cells, seem to have marked some of the neurons (in red) of my epileptic TSC FFPE human cortex tissue.
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