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  • KD/KO Validated

KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 21017-1-AP

Synonyms

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HT-1080 cells, human testis tissue, mouse colon tissue, mouse small intestine tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21017-1-AP is KLF5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15267
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC042131
Gene symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.

Protocols

Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-APDownload protocol
IHC protocol for KLF5 antibody 21017-1-APDownload protocol
IF protocol for KLF5 antibody 21017-1-APDownload protocol
IP protocol for KLF5 antibody 21017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Biochem Biophys Res Commun

miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.

Authors - Jian-Zhong Zhang
mouseIHC

Biomed Pharmacother

mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.

Authors - Wei Wang
ratWB

Cell Death Dis

The long non-coding RNA TUG1-miR-9a-5p axis contributes to ischemic injuries by promoting cardiomyocyte apoptosis via targeting KLF5.

Authors - Di Yang
humanWB,IHC

Cancers (Basel)

KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.

Authors - Juan Li
  • KD Validated
humanWB,ChIP

Mol Biol Rep

Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.

Authors - Xi Chen
  • KD Validated
humanWB

Perit Dial Int

An inhibitor of Krüppel-like factor 5 suppresses peritoneal fibrosis in mice.

Authors - Kumiko Muta