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  • KD/KO Validated

KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21017-1-AP


BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21017-1-AP is KLF5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15267
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC042131
Gene symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.


Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-APDownload protocol
IHC protocol for KLF5 antibody 21017-1-APDownload protocol
IF protocol for KLF5 antibody 21017-1-APDownload protocol
IP protocol for KLF5 antibody 21017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.

Authors - Jian-Zhong Zhang

Biomed Pharmacother

mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.

Authors - Wei Wang

Cell Death Dis

The long non-coding RNA TUG1-miR-9a-5p axis contributes to ischemic injuries by promoting cardiomyocyte apoptosis via targeting KLF5.

Authors - Di Yang

Cancers (Basel)

KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.

Authors - Juan Li
  • KD Validated

Cell Rep

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Authors - Wen-Shuai Tang

Methods Mol Biol

Evaluation of Barrier Functions in Human iPSC-Derived Intestinal Epithelium.

Authors - Shigeru Yamada