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KLF5 Polyclonal antibody
KLF5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 21017-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 14 publications below |
WB | See 37 publications below |
IHC | See 12 publications below |
IF | See 11 publications below |
IP | See 1 publications below |
CoIP | See 4 publications below |
ChIP | See 6 publications below |
Product Information
21017-1-AP targets KLF5 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | KLF5 fusion protein Ag15267 |
Full Name | Kruppel-like factor 5 (intestinal) |
Calculated Molecular Weight | 457 aa, 51 kDa |
Observed Molecular Weight | 51-55 kDa |
GenBank Accession Number | BC042131 |
Gene Symbol | KLF5 |
Gene ID (NCBI) | 688 |
RRID | AB_10696447 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).
Protocols
Product Specific Protocols | |
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WB protocol for KLF5 antibody 21017-1-AP | Download protocol |
IHC protocol for KLF5 antibody 21017-1-AP | Download protocol |
IF protocol for KLF5 antibody 21017-1-AP | Download protocol |
IP protocol for KLF5 antibody 21017-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. | ||
Oncogene KLF5 regulates actin remodeling to enhance the metastasis of nasopharyngeal carcinoma | ||
Cell Death Dis DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.
| ||
Aging (Albany NY) Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.
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Oncotarget Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Sarah-Eve (Verified Customer) (03-01-2023) | Works okay on pvdf membrane, blocking with skim milk. A lot of bands.
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