|Positive WB detected in||HeLa cells, A549 cells, HT-1080 cells, human testis tissue, mouse colon tissue, mouse small intestine tissue, PC-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse embryo tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21017-1-AP is KLF5 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF5 fusion protein Ag15267|
|Full Name||Kruppel-like factor 5 (intestinal)|
|Calculated molecular weight||457 aa, 51 kDa|
|Observed molecular weight||51-55 kDa|
|GenBank accession number||BC042131|
|Gene ID (NCBI)||688|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too.
|Product Specific Protocols|
|WB protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IHC protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IF protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IP protocol for KLF5 antibody 21017-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
miR-448-3p controls intracranial aneurysm by regulating KLF5 expression.
mircroRNA-152 prevents the malignant progression of atherosclerosis via down-regulation of KLF5.
Cell Death Dis
The long non-coding RNA TUG1-miR-9a-5p axis contributes to ischemic injuries by promoting cardiomyocyte apoptosis via targeting KLF5.
KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer Cells.
Mol Biol Rep
Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.
Perit Dial Int
An inhibitor of Krüppel-like factor 5 suppresses peritoneal fibrosis in mice.