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  • KD/KO Validated

KLF5 Polyclonal antibody

KLF5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21017-1-AP


BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

Tested Applications

Positive WB detected inA549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21017-1-AP targets KLF5 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15267
Full Name Kruppel-like factor 5 (intestinal)
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).


Product Specific Protocols
WB protocol for KLF5 antibody 21017-1-APDownload protocol
IHC protocol for KLF5 antibody 21017-1-APDownload protocol
IF protocol for KLF5 antibody 21017-1-APDownload protocol
IP protocol for KLF5 antibody 21017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Integrated multi-omics analyses identify anti-viral host factors and pathways controlling SARS-CoV-2 infection

Authors - Jiakai Hou

Cell Res

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Authors - Shunrong Ji

Cell Death Dis

DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.

Authors - Rong-Jie Fu
  • KD Validated

Aging (Albany NY)

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

Authors - Yilong Li
  • KD Validated


Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Authors - Xiaolong Wei
  • KD Validated

Eur J Pharmacol

Early growth response 1/Krüppel-like factor 5 pathway inhibitor alleviates lipopolysaccharide-induced lung injury by promoting autophagy

Authors - Kang Zou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah-Eve (Verified Customer) (03-01-2023)

Works okay on pvdf membrane, blocking with skim milk. A lot of bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Human pulmonary smooth muscle cells
KLF5 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary smooth muscle cells (Cat no:21017-1-AP)