Validation Data Gallery
|Positive WB detected in||A549 cells, HeLa cells, Caco-2 cells, mouse colon tissue, human testis tissue, PC-3 cells, HT-1080 cells, mouse small intestine tissue, HCT 116 cells, COLO 320 cells, rat colon tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse embryo tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
21017-1-AP targets KLF5 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF5 fusion protein Ag15267|
|Full Name||Kruppel-like factor 5 (intestinal)|
|Calculated molecular weight||457 aa, 51 kDa|
|Observed molecular weight||51-55 kDa, 85-120 kDa|
|GenBank accession number||BC042131|
|Gene ID (NCBI)||688|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling. This antibody detects the 50-60 kDa isoform 1 of KLF5. The antibody can recognize isoform 2 (33 kDa) and isoform 3 (18 kDa) too. 85 kDa, 95 kDa and 120 kDa of SUMOylated KLF5 were detected (PMID: 18500350).
|Product Specific Protocols|
|WB protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IHC protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IF protocol for KLF5 antibody 21017-1-AP||Download protocol|
|IP protocol for KLF5 antibody 21017-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.
Cell Death Dis
DNMT1-maintained hypermethylation of Krüppel-like factor 5 involves in the progression of clear cell renal cell carcinoma.
Aging (Albany NY)
Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.
Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.
A Weighted Gene Co-Expression Network Analysis-Derived Prognostic Model for Predicting Prognosis and Immune Infiltration in Gastric Cancer.
Identification and Characterization of TF-lncRNA Regulatory Networks Involved in the Tumorigenesis and Development of Adamantinomatous Craniopharyngioma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah-Eve (Verified Customer) (03-01-2023)
Works okay on pvdf membrane, blocking with skim milk. A lot of bands.