KLF6 Polyclonal antibody

KLF6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14716-1-AP

Synonyms

B cell derived protein 1, COPEB, CPBP, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, mouse colon tissue, mouse testis tissue, mouse thymus tissue, PC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14716-1-AP targets KLF6 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF6 fusion protein Ag6440
Full Name Kruppel-like factor 6
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa;42-45 kDa
GenBank accession numberBC000311
Gene symbol KLF6
Gene ID (NCBI) 1316
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

Protocols

Product Specific Protocols
WB protocol for KLF6 antibody 14716-1-APDownload protocol
IHC protocol for KLF6 antibody 14716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Development

Postnatal liver functional maturation requires Cnot complex-mediated decay of mRNAs encoding cell cycle and immature liver genes.

Authors - Toru Suzuki
humanWB

Cell Death Dis

Artesunate induces mitochondria-mediated apoptosis of human retinoblastoma cells by upregulating Kruppel-like factor 6.

Authors - Ying Yang
humanWB

Cell Death Dis

Hotspot mutant p53-R273H inhibits KLF6 expression to promote cell migration and tumor metastasis.

Authors - Shengnan Sun
mouseWB,IF

Front Physiol

MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.

Authors - Yue Zhang
mouseWB

J Ethnopharmacol

Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.

Authors - Jing Zhou
humanWB

Nutrients

The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression.

Authors - Yue Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paulo (Verified Customer) (03-01-2019)

Used to perform ChIP-seq in ccRCC cell lines. 20ug of antibody used in 100million cells. No peaks were detected in known target genes and obtained peaks failed to present the consensus KLF6 binding site after motif analysis.

  • Applications: ChIP,