KLF6 Polyclonal antibody

KLF6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (1)

Cat no : 14716-1-AP

Synonyms

B cell derived protein 1, COPEB, CPBP, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9

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Validation Data Gallery

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  WB analysis using 14716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14716-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse colon using 14716-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse thymus using 14716-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14716-1-AP (KLF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 14716-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14716-1-AP (KLF6 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14716-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14716-1-AP (KLF6 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse thymus tissue, mouse colon tissue, HUVEC cells, NCI-H1299 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

14716-1-AP targets KLF6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF6 fusion protein Ag6440
• Full Name: Kruppel-like factor 6
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-42 kDa
• GenBank Accession Number: BC000311
• Gene Symbol: KLF6
• Gene ID (NCBI): 1316
• RRID: AB_10640526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

Publications

Species	Application	Title
human,mouse	WB,IF	Adv Sci (Weinh); Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6; Authors - Chao Wei
human	WB	Nutrients; The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression.; Authors - Yue Wang
mouse	WB	J Ethnopharmacol; Salvia miltiorrhiza bunge exerts anti-oxidative effects through inhibiting KLF10 expression in vascular smooth muscle cells exposed to high glucose.; Authors - Jing Zhou
mouse	IF	Front Genet; Revealing Potential Spinal Cord Injury Biomarkers and Immune Cell Infiltration Characteristics in Mice.; Authors - Liang Cao
mouse	WB,IF	Front Physiol; MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.; Authors - Yue Zhang
rat	WB	Bioengineered; MicroRNA-124 modulates neuroinflammation in acute methanol poisoning rats via targeting Krüppel-like factor-6.; Authors - Shu Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paulo (Verified Customer) (03-01-2019)

Used to perform ChIP-seq in ccRCC cell lines. 20ug of antibody used in 100million cells. No peaks were detected in known target genes and obtained peaks failed to present the consensus KLF6 binding site after motif analysis.

• Applications: ChIP,