PPP1R21 Polyclonal antibody

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  WB analysis using 31532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31532-1-AP (KLRAQ1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 31532-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31532-1-AP (PPP1R21 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 31532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using KLRAQ1 antibody (31532-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP1R21 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 31532-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

KLRAQ1, NEDHFBA, KLRAQ motif containing 1, FERRY2, CCDC128

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Tested Applications

• Positive WB detected in: MCF-7 cells, THP-1 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31532-1-AP targets PPP1R21 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPP1R21 fusion protein Ag35449
• Full Name: KLRAQ motif containing 1
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC111059
• Gene Symbol: KLRAQ1
• Gene ID (NCBI): 129285
• RRID: AB_3670023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6ZMI0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R21, also known as KLRAQ1, CCDC12 and FERRY2, encodes a conserved protein involved in endosomal maturation. It has many isoforms and may play a role in the endosomal sorting process or in the endosome maturation pathway.