Cytokeratin 19 Polyclonal antibody
Cytokeratin 19 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10712-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, HepG2 cells, MCF-7 cells, mouse brain tissue, mouse placenta tissue |
| Positive IHC detected in | human colon tissue, mouse skin tissue, mouse colon tissue, human stomach cancer tissue, human lung cancer tissue, human skin tissue, human liver cancer tissue, rat liver tissue, human liver tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:6400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 9 publications below |
| IHC | See 28 publications below |
| IF | See 22 publications below |
Product Information
10712-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 19 fusion protein Ag1085 |
| Full Name | keratin 19 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 44-50 kDa |
| GenBank accession number | BC007628 |
| Gene symbol | KRT19 |
| Gene ID (NCBI) | 3880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 19 antibody 10712-1-AP | Download protocol |
| IHC protocol for Cytokeratin 19 antibody 10712-1-AP | Download protocol |
| IF protocol for Cytokeratin 19 antibody 10712-1-AP | Download protocol |
| FC protocol for Cytokeratin 19 antibody 10712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dis Model Mech A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. | ||
Vaccines (Basel) Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas. | ||
Stem Cells Int Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway. |
