Validation Data Gallery
|Positive WB detected in||A431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells|
|Positive IHC detected in||human colon tissue, human pancreas tissue, human thyroid cancer tissue, mouse liver tissue, human oesophagus cancer tissue, mouse skin tissue, mouse colon tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:3000-1:30000|
|Immunohistochemistry (IHC)||IHC : 1:3000-1:12000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10712-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||dog, goat, human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 19 fusion protein Ag1085|
|Full Name||keratin 19|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||44-50 kDa|
|GenBank accession number||BC007628|
|Gene ID (NCBI)||3880|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.
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