Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

84

Cat no : 10712-1-AP

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

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Validation Data Gallery

• 

  WB analysis using 10712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10712-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10712-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10712-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10712-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human oesophagus cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse colon using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human stomach cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human pancreas using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human lung cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse liver using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of mouse liver using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human thyroid cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human breast cancer using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IHC staining of human skin using 10712-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10712-1-AP (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens).

  Ready-to-use IHC kits available with KRT19 antibody included
• 

  IF Staining of HeLa using 10712-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 19 antibody (10712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10712-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 19 antibody (10712-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells
• Positive IHC detected in: human colon tissue, human pancreas tissue, human thyroid cancer tissue, mouse liver tissue, human oesophagus cancer tissue, mouse skin tissue, mouse colon tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: MCF-7 cells

Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.

Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:30000
• Immunohistochemistry (IHC): IHC : 1:3000-1:12000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 16 publications below
• IHC: See 42 publications below
• IF: See 38 publications below

Product Information

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: dog, goat, human, mouse, pig, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 19 fusion protein Ag1085
• Full Name: keratin 19
• Calculated molecular weight: 40 kDa
• Observed molecular weight: 44-50 kDa
• GenBank accession number: BC007628
• Gene symbol: KRT19
• Gene ID (NCBI): 3880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Publications

Species	Application	Title
human	IHC	Oncol Lett; Cytokeratin expression in epidermal stem cells in skin adnexal tumors.; Authors - Xiaoqiu Zhou
human	IHC	Oncogene; THZ1 reveals CDK7-dependent transcriptional addictions in pancreatic cancer.; Authors - Ping Lu
	IF	Dis Model Mech; A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal.; Authors - Qiu Tu
human	IF	Vaccines (Basel); Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas.; Authors - Takako Tabata
rat	IF	Med Sci Monit; PMID: 31929497; Authors - Fang Xu
human	IHC,IF	Stem Cells Int; Human Adipose-Derived Stem Cells Promote Seawater-Immersed Wound Healing by Activating Skin Stem Cells via the EGFR/MEK/ERK Pathway.; Authors - Jiachao Xiong

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