Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10712-1-AP

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive WB detected inA431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue, human thyroid cancer tissue, mouse liver tissue, human oesophagus cancer tissue, mouse skin tissue, mouse colon tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:30000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10712-1-AP targets Cytokeratin 19 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, dog, goat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag1085
Full Name keratin 19
Calculated molecular weight 40 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC007628
Gene symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
human

Adv Sci (Weinh)

PMID: 31728273

Authors - Qingming Tang
human

Nat Commun

Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.

Authors - Xuexiang Han
humanIHC

J Clin Invest

CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.

Authors - Ki-Hyun Kim

Cell Host Microbe

Zika Virus Targets Different Primary Human Placental Cells, Suggesting Two Routes for Vertical Transmission.

Authors - Takako Tabata
humanWB

Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: ROs