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Cytokeratin 19 Antibody 40 Publications

Rabbit Polyclonal| Catalog number: 10712-1-AP

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

Cytokeratin 19 Antibody WB A431 cells 10712-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 10712-1-AP(KRT19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

Cytokeratin 19 Antibody IHC human colon tissue 10712-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 19 Antibody IHC human colon tissue 10712-1-AP

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:6400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 19 Antibody IF HepG2 cells 10712-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Cytokeratin 19 Antibody FC MCF-7 cells 10712-1-AP

1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 19 antibody (10712-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, A549 cells, HepG2 cells, MCF-7 cells, mouse brain tissue, mouse placenta tissue
Positive IHC detected in:
human colon tissue, rat liver tissue, human liver cancer tissue, human liver tissue, human pancreas tissue, human lung cancer tissue, human stomach cancer tissue, human skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:3000
IHC : 1:1000-1:6400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 19 fusion protein Ag1085
Full name:
keratin 19
Calculated molecular weight:
40 kDa
Observed molecular weight:
44-50 kDa
GenBank accession number:
BC007628
Gene ID (NCBI):
3880
Gene symbol
KRT19
Synonyms
CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.


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