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Cytokeratin 19 Antibody 1 Publications

Mouse Monoclonal| Catalog number: 60187-1-Ig |CloneNo.: 3G1E4

Featured Product KD/KO validated

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Con: 170 μg/150 μl

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Tested Applications
Applications:
ELISA, IHC, WB
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Images See all

Cytokeratin 19 Antibody WB MCF-7 cells 60187-1-Ig

MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig( Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

Cytokeratin 19 Antibody WB HeLa cells 60187-1-Ig

WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

Cytokeratin 19 Antibody IHC human stomach cancer tissue 60187-1-Ig

Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig( Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Cytokeratin 19 Antibody IHC human stomach cancer tissue 60187-1-Ig

Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig( Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
MCF-7 cells,HeLa cells, HepG2 cells
Positive IHC detected in:
human stomach cancer tissue,human lung cancer tissue, human breast cancer tissue
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 19 fusion protein Ag7407
Full name:
keratin 19
Calculated molecular weight:
44 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
BC002539
Gene ID (NCBI):
3880
Gene symbol
KRT19
Synonyms
CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
Background

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.


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