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- KD/KO Validated
Cytokeratin 19 Monoclonal antibody
Cytokeratin 19 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, IF
Conjugate
Unconjugated
CloneNo.
3G1E4
Cat no : 60187-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IHC detected in | human colon tissue, human oesophagus cancer tissue, human breast cancer tissue, human thyroid cancer tissue, human lung cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:51200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
60187-1-Ig targets Cytokeratin 19 in WB, IHC, IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cytokeratin 19 fusion protein Ag7407 |
| Full Name | keratin 19 |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC002539 |
| Gene symbol | KRT19 |
| Gene ID (NCBI) | 3880 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IF protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reprod Sci Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis | ||
Biochim Biophys Acta Mol Basis Dis TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis | ||
Biomed Pharmacother Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis. | ||
Stem Cells Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells. |
