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Cytokeratin 19 Monoclonal antibody

Cytokeratin 19 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat, pig







Cat no : 60187-1-Ig


Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19

Tested Applications

Positive WB detected inMCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human thyroid cancer tissue, human stomach cancer tissue, human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:51200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60187-1-Ig targets Cytokeratin 19 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag7407
Full Name keratin 19
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC002539
Gene Symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.


Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
IHC protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
IF protocol for Cytokeratin 19 antibody 60187-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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