Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65128-1-Ig targets IgG2b Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | immunoglobulin heavy chain 3 (serum IgG2b) |
| Gene Symbol | IgG2b Isotype Control |
| Gene ID (NCBI) | 16016 |
| RRID | AB_2918419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01867 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The MPC-11 immunoglobulin is useful as an isotype-matched control. The MPC-11 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2b Isotype Control antibody 65128-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Mouse IgG2b Isotype Control (MPC-11), catalog number 65128-1-Ig, has 1 citation. It appears in iScience (IF 4.5). The associated research field is intestinal immunology and metabolism, specifically investigating how live and heat-treated Lactiplantibacillus plantarum induce distinct metabolic and immune responses in intestinal epithelial cells.
| Species | Application | Title |
|---|---|---|
iScience Live and heat-treated Lactiplantibacillus plantarum induce distinct metabolic and immune responses in intestinal epithelial cells. |
Reviews
What customers say
The single review for 65128-1-Ig is from a user who applied it in Flow Cytometry on cancer cells. They gave it a 5-star rating, praising the fast delivery, great quality, and affordability. The user noted that the secondary antibody was handled under the same conditions as the primary antibody. Overall, the feedback is positive with no reported issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cyril (Verified Customer) (12-08-2025) | Fast delivery, great quality, and very affordable.
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