|Positive WB detected in||MCF-7 cells, A431 cells, A549 cells, C6 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||hamster, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||keratin 8|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||NM_002273|
|Gene ID (NCBI)||3856|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
|Product Specific Protocols|
|WB protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|IHC protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|IF protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|FC protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
This antibody recognizes a band that matched with the expected molecular weight of KRT8.