Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17514-1-AP

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8



Tested Applications

Positive WB detected inA431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002273
Gene symbol KRT8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
FC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
mouseIHC

Sci Rep

Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators.

Authors - Shu Wei
mouseWB,IF

iScience

Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid.

Authors - Jianjun Zhao
mouseIHC

Front Med (Lausanne)

An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.

Authors - Songyan Gao
human, hamsterWB

Cancer Gene Ther

Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.

Authors - S Vijayalingam

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)
FH

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)