Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse and More (1)





Cat no : 17514-1-AP


CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

Tested Applications

Positive WB detected inA431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002273
Gene symbol KRT8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.


Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
FC protocol for Cytokeratin 8 antibody 17514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Exp Med

Wnt5a induces and maintains prostate cancer cells dormancy in bone.

Authors - Dong Ren

Oncol Lett

Cytokeratin expression in epidermal stem cells in skin adnexal tumors.

Authors - Xiaoqiu Zhou

Biochem Biophys Rep

KLF4 transactivates TRIM29 expression and modulates keratin network.

Authors - Runqing Huang

Signal Transduct Target Ther

Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma.

Authors - Jiajun Xie

Exp Ther Med

Protective effects of miR-155-5p silencing on IFN-γ-induced apoptosis and inflammation in salivary gland epithelial cells.

Authors - Jingli Zhang

J Mol Histol

Isolation, culture, and identification of ceruminous gland cells.

Authors - Jun Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Emma (Verified Customer) (11-29-2021)

Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
Cytokeratin 8 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17514-1-AP)

Joleen (Verified Customer) (06-12-2019)

This antibody recognizes a band that matched with the expected molecular weight of KRT8.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Cytokeratin 8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:17514-1-AP)