Validation Data Gallery
|Positive WB detected in||MCF-7 cells, A431 cells, A549 cells, C6 cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, human colon tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||hamster, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||keratin 8|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||NM_002273|
|Gene ID (NCBI)||3856|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
|Product Specific Protocols|
|WB protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|IHC protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|IF protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|FC protocol for Cytokeratin 8 antibody 17514-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Med
Wnt5a induces and maintains prostate cancer cells dormancy in bone.
Cytokeratin expression in epidermal stem cells in skin adnexal tumors.
Biochem Biophys Rep
KLF4 transactivates TRIM29 expression and modulates keratin network.
Exp Ther Med
Protective effects of miR-155-5p silencing on IFN-γ-induced apoptosis and inflammation in salivary gland epithelial cells.
J Cancer Res Clin Oncol
Animal and cellular models of hepatocellular carcinoma bone metastasis: establishment and characterisation.
Cancer Gene Ther
Evaluation of apoptogenic adenovirus type 5 oncolytic vectors in a Syrian hamster head and neck cancer model.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (06-12-2019)
This antibody recognizes a band that matched with the expected molecular weight of KRT8.