Tested Applications
| Positive WB detected in | A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human appendicitis tissue, human bowen disease tissue, human colon tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon tissue, mouse testis tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, hamster, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | keratin 8 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | NM_002273 |
| Gene Symbol | Cytokeratin 8 |
| Gene ID (NCBI) | 3856 |
| RRID | AB_2134726 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IF protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IHC protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| WB protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytokeratin 8 Polyclonal antibody (Cat# 17514-1-AP) has 40 citations. They appear in journals including Signal Transduction and Targeted Therapy, Nature Biomedical Engineering, Nature Aging, Nature Communications, Oncogene, Journal of Experimental Medicine, Clinical & Translational Medicine, Frontiers in Immunology, PLoS Pathogens, and Scientific Reports. Research fields span cancer biology (prostate, gastric, pancreatic, lung, cervical, esophageal, hepatocellular carcinoma), epithelial biology and EMT, immunology, organoid models, developmental biology, and comparative/veterinary studies across human, mouse, rat, sheep, zebrafish, and other species.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma. | ||
Nat Biomed Eng Mammary organoid-based depot for post-surgical chemotherapy and gland regeneration. | ||
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Nat Commun Actomyosin forces trigger a conformational change in desmoplakin within desmosomes. | ||
J Adv Res METTL4 enhances GLI1 translation through m(6)Am modification to promote tumor progression as a therapeutic target for hepatocellular carcinoma. | ||
Cell Mol Biol Lett OTULIN protects hyperoxia-induced neonatal lung injury and modulates mitochondrial protein OPA1 in association with the E3 ubiquitin ligase RNF31. |
Reviews
What customers say
All three reviews are 5-star ratings. Users report excellent performance across multiple applications: IHC on human lung cancer tissue at 1:1000 with DAB staining, IF on FFPE prostate tissue at 1:50 with Tris-EDTA antigen retrieval, and Western blot on MDCK cells at 1:1000, where the antibody recognized a band matching the expected molecular weight of KRT8. Reviewers consistently describe the antibody as working well and highly recommend it.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nikolett (Verified Customer) (03-31-2026) | Antibody works well on human lung cancer patient-derived tissue as 1:1000 for DAB IHC staining under pH9 TE buffer conditions. I highly recommend it.
|
Emma (Verified Customer) (11-29-2021) | Works very well by IF on FFPE tissue. Used at 1:50 with a Tris-EDTA antigen retrieval.
 |
Joleen (Verified Customer) (06-12-2019) | This antibody recognizes a band that matched with the expected molecular weight of KRT8.
 |
