Tested Applications
Positive WB detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31197-1-AP targets Keap1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Keap1 fusion protein Ag35035 Predict reactive species |
Full Name | kelch-like ECH-associated protein 1 |
Calculated Molecular Weight | 70 kDa |
Observed Molecular Weight | 60-64 kDa |
GenBank Accession Number | NM_001110305 |
Gene Symbol | Keap1 |
Gene ID (NCBI) | 50868 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q9Z2X8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KEAP1 (Kelch-like ECH-associated protein 1) is a critical regulatory protein that plays a pivotal role in the cellular response to oxidative stress. It is best known for its function as a negative regulator of the Nrf2 (NF-E2-related factor 2) pathway, which is essential for maintaining cellular redox homeostasis.
Protocols
Product Specific Protocols | |
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WB protocol for Keap1 antibody 31197-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |