Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, mouse heart tissue, K-562 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse liver tissue, human heart tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 12 publications below |
| IHC | See 10 publications below |
| IF | See 8 publications below |
| IP | See 2 publications below |
Product Information
20966-1-AP targets LEPR in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | leptin receptor |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 100-150 kDa |
| GenBank Accession Number | NM_002303 |
| Gene Symbol | LEPR |
| Gene ID (NCBI) | 3953 |
| RRID | AB_2878775 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48357 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described. This antibody can recognize long (LEPRb) and short (LEPRa,c,d) isoforms. The band at 150 kDa detected by this antibody is larger than the predicted molecular weight, probably due to glycosylation (PMID: 10026237).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LEPR antibody 20966-1-AP | Download protocol |
| IHC protocol for LEPR antibody 20966-1-AP | Download protocol |
| IF protocol for LEPR antibody 20966-1-AP | Download protocol |
| IP protocol for LEPR antibody 20966-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer | ||
Oncotarget Leptin signaling enhances cell invasion and promotes the metastasis of human pancreatic cancer via increasing MMP-13 production. | ||
Cancers (Basel) Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression. | ||
Comput Struct Biotechnol J Celastrol inhibits the proliferation and migration of MCF-7 cells through the leptin-triggered PI3K/AKT pathway. | ||
FASEB J Immunotherapy against angiotensin II receptor ameliorated insulin resistance in a leptin receptor-dependent manner.
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