ILT-3/LILRB4 Monoclonal antibody

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  WB analysis of MCF-7 using 67119-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67119-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67119-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67119-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LILRB4 antibody (67119-1-Ig, Clone: 1C8E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ILT-3/LILRB4 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67119-1-Ig

Clone No.1C8E1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, HUVEC cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67119-1-Ig targets ILT-3/LILRB4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3219

  Product name: Recombinant human LILRB4 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 18-262 aa of BC026309

  Sequence: RTDMQAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEGPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWEVLI

• Full Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
• Calculated Molecular Weight: 448 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC026309
• Gene Symbol: LILRB4
• Gene ID (NCBI): 11006
• RRID: AB_2882422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NHJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.