LIMS1 Polyclonal antibody

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  WB analysis of HeLa using 20772-1-AP

  WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.

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  WB analysis using 20772-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 20772-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20772-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20772-1-AP

  IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:800) with K-562 cells lysate 1640 ug.

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  IHC staining of human colon cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20772-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20772-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIMS1 antibody (20772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 20772-1-AP

  Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20772-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

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Tested Applications

• Positive WB detected in: Caco-2 cells, human kidney tissue, HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Product Information

20772-1-AP targets LIMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14764

  Product name: Recombinant human LIMS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC005341

  Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK

• Full Name: LIM and senescent cell antigen-like domains 1
• Calculated Molecular Weight: 325 aa, 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC005341
• Gene Symbol: LIMS1
• Gene ID (NCBI): 3987
• RRID: AB_10695765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48059
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo ; KO Validated
human	WB,IP	J Cell Biol; A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.; Authors - Ling Guo ; KD Validated
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
	WB	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui ; KD Validated
human	WB	Cancer Med; The RNA-binding protein RBMS3 inhibits the progression of colon cancer by regulating the stability of LIMS1 mRNA; Authors - Yafei Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody for western blot. Works well at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000 for western blot
• Cell Tissue Type: HeLa, NIH3T3