LIMS2 Polyclonal antibody

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  WB analysis using 55300-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55300-1-AP (LIMS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

LIMS2 Polyclonal Antibody for WB, ELISA

Cat No. 55300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LIM like protein 2, PINCH2, PINCH-2

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse bladder tissue, rat bladder tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55300-1-AP targets LIMS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LIM and senescent cell antigen-like domains 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_017980
• Gene Symbol: LIMS2
• Gene ID (NCBI): 55679
• RRID: AB_3670247
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z4I7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIMS2, also known as PINCH2, is a member of a small family of focal adhesion proteins. LIMS2 contains five LIM domains, each domain forming two zinc fingers, which permit interactions that regulate cell shape and migration. Along with integrin-linked kinase and parvin, LIMS2 is a component of a complex that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM) (PMID: 12167643). Mutations in LIMS2 are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues(PMID: 25589244).