LIX1L Polyclonal antibody

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  WB analysis using 25557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25557-1-AP (LIX1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 25557-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using LIX1L antibody (25557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LIX1L Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25557-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LIX1-like protein

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, SH-SY5Y cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25557-1-AP targets LIX1L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIX1L fusion protein Ag22209
• Full Name: Lix1 homolog (mouse)-like
• Calculated Molecular Weight: 337 aa, 37 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC035727
• Gene Symbol: LIX1L
• Gene ID (NCBI): 128077
• RRID: AB_3669484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVB5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Limb expression 1-like protein (LIX1L) is a putative RNA binding protein that contains a double-stranded RNA-binding motif. LIX1L is highly expressed in various cancer tissues.