LRRC2 Recombinant antibody

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  IHC staining of mouse skeletal muscle using 84404-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 84404-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 84404-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LRRC2 antibody (84404-1-RR, Clone: 241132G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-251 using 84404-1-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug LRRC2 Recombinant antibody (84404-1-RR, Clone:241132G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84404-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84404-1-RR against Human LRRC2 were performed. The affinity constant is 26.2 nM.

LRRC2 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 84404-1-RR

Clone No.241132G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Leucine-rich repeat-containing protein 2, 241132G1

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Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84404-1-RR targets LRRC2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRC2 fusion protein Ag27830
• Full Name: leucine rich repeat containing 2
• Calculated Molecular Weight: 371 aa, 43 kDa
• GenBank Accession Number: BC029118
• Gene Symbol: LRRC2
• Gene ID (NCBI): 79442
• RRID: AB_3671933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9BYS8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)