LYN Polyclonal antibody

LYN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 18135-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

EC:2.7.10.2, JTK8, Lck/Yes-related novel protein tyrosine kinase, Tyrosine protein kinase Lyn, Tyrosine-protein kinase Lyn

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18135-1-AP targets LYN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12723

Product name: Recombinant human LYN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 132-512 aa of BC075002

Sequence: KDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP

Predict reactive species
Full Name v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Calculated Molecular Weight 512 aa, 59 kDa
Observed Molecular Weight 53-56 kDa, 66-70 kDa
GenBank Accession NumberBC075002
Gene Symbol LYN
Gene ID (NCBI) 4067
RRIDAB_11042319
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Protocols

Product Specific Protocols
WB protocol for LYN antibody 18135-1-APDownload protocol
IHC protocol for LYN antibody 18135-1-APDownload protocol
IF protocol for LYN antibody 18135-1-APDownload protocol
IP protocol for LYN antibody 18135-1-APDownload protocol
FC protocol for LYN antibody 18135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Invest Dermatol

Loss of LYN is frequent in targeted-therapy resistant melanoma cells and favors metastatic properties

Authors - Céline Gaudel
  • KD Validated
humanIHC

Front Cell Dev Biol

Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.

Authors - Chonghua Jiang
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