Validation Data Gallery
|Positive WB detected in
|HeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
|Positive IP detected in
|Positive IHC detected in
|human kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:20-1:200
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 18135-1-AP is LYN Fusion Protein expressed in E. coli.
|Host / Isotype
|Rabbit / IgG
|LYN fusion protein Ag12723
|v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
|Calculated molecular weight
|512 aa, 59 kDa
|Observed molecular weight
|53-56 kDa, 66-70 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
|Product Specific Protocols
|WB protocol for LYN antibody 18135-1-AP
|IHC protocol for LYN antibody 18135-1-AP
|IF protocol for LYN antibody 18135-1-AP
|IP protocol for LYN antibody 18135-1-AP
|FC protocol for LYN antibody 18135-1-AP
|Click here to view our Standard Protocols