Lamin B1 Polyclonal antibody, PBS Only

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  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12987-1-AP (same clone as 12987-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 12987-1-AP (same clone as 12987-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse eye using 12987-1-AP (same clone as 12987-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 12987-1-AP (same clone as 12987-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 12987-1-AP (same clone as 12987-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 12987-1-AP (same clone as 12987-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 12987-1-AP (same clone as 12987-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 12987-1-AP (same clone as 12987-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12987-1-AP (LMNB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12987-1-AP (same clone as 12987-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12987-1-AP (same clone as 12987-1-PBS)

  IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:20000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human skin cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 12987-1-AP (same clone as 12987-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Lamin B1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Cat No. 12987-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Synonyms

LMNB1, laminB1, Lamin-B1, LMN2, LMNB

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Product Information

12987-1-PBS targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lamin B1 fusion protein Ag3631
• Full Name: lamin B1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC012295
• Gene Symbol: Lamin B1
• Gene ID (NCBI): 4001
• ENSEMBL Gene ID: ENSG00000113368
• RRID: AB_2136290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20700
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.