Lamin B1 Polyclonal antibody

Lamin B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, FC, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 12987-1-AP

Synonyms

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1



Tested Applications

Positive WB detected inHeLa cells, U-937 cells, mouse spleen tissue, mouse pancreas tissue, mouse lung tissue, SH-SY5Y cells, K-562 cells, Y79 cells, mouse eye tissue, mouse kidney tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human skin cancer tissue, NIH/3T3 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12987-1-AP targets Lamin B1 in WB, IP, IHC, IF, FC, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, chicken, hamster, human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lamin B1 fusion protein Ag3631
Full Name lamin B1
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC012295
Gene symbol LMNB1
Gene ID (NCBI) 4001
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Product Specific Protocols
WB protocol for Lamin B1 antibody 12987-1-APDownload protocol
IHC protocol for Lamin B1 antibody 12987-1-APDownload protocol
IF protocol for Lamin B1 antibody 12987-1-APDownload protocol
IP protocol for Lamin B1 antibody 12987-1-APDownload protocol
FC protocol for Lamin B1 antibody 12987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
humanWB

EBioMedicine

Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway.

Authors - Binghua Li
mouseWB

J Agric Food Chem

PMID: 30592218

Authors - Yao Liu
humanWB

J Cell Mol Med

Knockdown of Golgi phosphoprotein 73 blocks the trafficking of matrix metalloproteinase-2 in hepatocellular carcinoma cells and inhibits cell invasion.

Authors - Yiming Liu
ratWB

Naunyn Schmiedebergs Arch Pharmacol

PMID: 30683943

Authors - Wei Feng
humanWB

PLoS One

Fluctuations in cell density alter protein markers of multiple cellular compartments, confounding experimental outcomes.

Authors - Katarina Trajkovic

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-04-2020)

Very sharp bands for nuclear protein

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000 for western
  • Cell Tissue Type: Human colon cancer
FH

Chun (Verified Customer) (12-05-2019)

Excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: HEK293 and HaCaT cells
FH

Marco (Verified Customer) (09-02-2019)

good staining in conjunction with Alexa Fluor Plus 488 secondary

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human mesenchymal stem cells