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  • KD/KO Validated

MAD2L2 Polyclonal antibody

MAD2L2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12683-1-AP


hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog

Tested Applications

Positive WB detected inhuman brain tissue, A375 cells, fetal HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12683-1-AP targets MAD2L2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag3373
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.


Product Specific Protocols
WB protocol for MAD2L2 antibody 12683-1-APDownload protocol
IHC protocol for MAD2L2 antibody 12683-1-APDownload protocol
IP protocol for MAD2L2 antibody 12683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.

Authors - Inge de Krijger
  • KO Validated

Nat Commun

SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.

Authors - Estelle Vincendeau
humanWB, IP

J Cell Biol

Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.

Authors - Tamar Listovsky
  • KD Validated

Nucleic Acids Res

REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ

Authors - Junya Tomida
  • KD Validated

Nucleic Acids Res

REV1 and polymerase ζ facilitate homologous recombination repair.

Authors - Sharma Shilpy S
  • KD Validated

Nucleic Acids Res

Regulation of error-prone translesion synthesis by Spartan/C1orf124.

Authors - Kim Myoung Shin MS