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  • KD/KO Validated

MAD2L2 Polyclonal antibody

MAD2L2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12683-1-AP

Synonyms

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog



Tested Applications

Positive WB detected inhuman brain tissue, A375 cells, fetal HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12683-1-AP targets MAD2L2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag3373
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated molecular weight 211 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC015244
Gene symbol MAD2L2
Gene ID (NCBI) 10459
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Protocols

Product Specific Protocols
WB protocol for MAD2L2 antibody 12683-1-APDownload protocol
IHC protocol for MAD2L2 antibody 12683-1-APDownload protocol
IP protocol for MAD2L2 antibody 12683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

Authors - Stephen Gray
humanWB

Biochem Biophys Res Commun

CDH1 binds MAD2L2 in a Rev1-like pattern.

Authors - Nomi Pernicone
humanWB,IHC

Mol Oncol

MAD2L2 inhibits colorectal cancer growth by promoting NCOA3 ubiquitination and degradation.

Authors - Yixin Li
  • KD Validated
humanWB

Nucleic Acids Res

REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ

Authors - Junya Tomida
  • KD Validated
humanWB

Nucleic Acids Res

REV1 and polymerase ζ facilitate homologous recombination repair.

Authors - Sharma Shilpy S
  • KD Validated
humanWB

Nucleic Acids Res

Regulation of error-prone translesion synthesis by Spartan/C1orf124.

Authors - Kim Myoung Shin MS