MAP3K1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP3K1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 19970-1-AP

Synonyms

MEKK, MEK kinase 1, MAPKKK1, MAPK/ERK kinase kinase 1, EC:2.7.11.25

---

Validation Data Gallery

• 

  WB analysis using 19970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19970-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 19970-1-AP

  IP result of anti-MAP3K1 (IP:19970-1-AP, 4ug; Detection:19970-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

• 

  IHC staining of human spleen using 19970-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 19970-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19970-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19970-1-AP (MAP3K1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 1 publications below

Product Information

19970-1-AP targets MAP3K1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase kinase 1
• Calculated Molecular Weight: 164 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: NM_005921
• Gene Symbol: MAP3K1
• Gene ID (NCBI): 4214
• RRID: AB_2878633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.

Publications

Species	Application	Title
human	WB	J Immunol Res; Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1; Authors - Zutong Li
human	WB	Int J Biol Sci; LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in colorectal cancer.; Authors - Rui Tang
human	WB	Int Immunopharmacol; Extracellular vesicles secreted from mesenchymal stem cells exert anti-apoptotic and anti-inflammatory effects via transmitting microRNA-18b in rats with diabetic retinopathy.; Authors - Zepeng Xu
mouse	WB,IF	Biomed Pharmacother; Xihuang pill promotes apoptosis of Treg cells in the tumor microenvironment in 4T1 mouse breast cancer by upregulating MEKK1/SEK1/JNK1/AP-1 pathway.; Authors - Liang Su
human	WB	Cell Death Dis; LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia.; Authors - Doudou Tang
mouse	WB	Clin Sci (Lond); USP4 deficiency exacerbates hepatic ischaemia/reperfusion injury via TAK1 signalling.; Authors - Jiangqiao Zhou