Tested Applications
| Positive WB detected in | mouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19970-1-AP targets MAP3K1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 1 |
| Calculated Molecular Weight | 164 kDa |
| Observed Molecular Weight | 164 kDa |
| GenBank Accession Number | NM_005921 |
| Gene Symbol | MAP3K1 |
| Gene ID (NCBI) | 4214 |
| RRID | AB_2878633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13233 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| IHC protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| IP protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| WB protocol for MAP3K1 antibody 19970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MAP3K1 (MEKK1) antibody, catalog number 19970-1-AP, has 18 citations published in journals including Cell, Current Biology, Cell Death & Disease, International Journal of Biological Sciences, NPJ Science of Food, Frontiers in Immunology, Clinical Science, and Biochemical and Biophysical Research Communications. Research fields span oncology (leukemia, colorectal cancer, hepatocellular carcinoma, glioblastoma, breast cancer), immunology, neuroscience, cardiology, and food allergy.
| Species | Application | Title |
|---|---|---|
Cell Death Dis LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia. | ||
Int J Biol Sci LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in colorectal cancer. | ||
NPJ Sci Food Synthetic food pigment sunset yellow potentiates food allergy via immune dysregulation and intestinal barrier dysfunction. | ||
Cell Mol Gastroenterol Hepatol HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation | ||
Curr Biol A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function |
