MAVS; VISA Monoclonal antibody

MAVS; VISA Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A8E9

Cat no : 66911-1-Ig

Synonyms

CARDIF, DKFZp547C224, DKFZp666M015, FLJ27482, FLJ35386, FLJ38051, FLJ41962, IPS 1, IPS1, KIAA1271, MAVS, VISA



Tested Applications

Positive WB detected inA431 cells, L02 cells, Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:550-1:2200
Sample-dependent, check data in validation data gallery

Product Information

66911-1-Ig targets MAVS; VISA in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MAVS; VISA fusion protein Ag5949
Full Name mitochondrial antiviral signaling protein
Calculated molecular weight 57 kDa
Observed molecular weight 70-75 kDa, 50-55 kDa
GenBank accession numberBC044952
Gene symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Protocols

Product Specific Protocols
WB protocol for MAVS; VISA antibody 66911-1-IgDownload protocol
IHC protocol for MAVS; VISA antibody 66911-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Virus Res

Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.

Authors - Qian Li
humanWB

J Virol

Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.

Authors - Yumei Han
humanWB

Front Immunol

Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.

Authors - Zhihai Zhou