MAVS; VISA Monoclonal antibody
MAVS; VISA Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human and More (2)
WB, IHC, IF, ELISA
Cat no : 66911-1-Ig
Validation Data Gallery
|Positive WB detected in||A431 cells, L02 cells, Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, THP-1 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:550-1:2200|
|Sample-dependent, check data in validation data gallery|
66911-1-Ig targets MAVS; VISA in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MAVS; VISA fusion protein Ag5949|
|Full Name||mitochondrial antiviral signaling protein|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||50-55 kDa, 70-75 kDa|
|GenBank accession number||BC044952|
|Gene ID (NCBI)||57506|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
|Product Specific Protocols|
|WB protocol for MAVS; VISA antibody 66911-1-Ig||Download protocol|
|IHC protocol for MAVS; VISA antibody 66911-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Immunother Cancer
Targeting KDM4C enhances CD8+ T cell mediated antitumor immunity by activating chemokine CXCL10 transcription in lung cancer.
Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.
Encephalomyocarditis virus abrogates the IFN-β signaling pathway via its structural protein VP2.
DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation
Host protein, HSP90β, antagonizes IFN-β signaling pathway and facilitates the proliferation of encephalomyocarditis virus in vitro.