MAVS; VISA Monoclonal antibody, PBS Only (Capture)

MAVS; VISA Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A8E9

Cat no : 66911-1-PBS

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

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Validation Data Gallery

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  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66911-1-Ig (same clone as 66911-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66911-1-Ig (same clone as 66911-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66911-1-Ig (same clone as 66911-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66911-1-Ig (same clone as 66911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66911-1-Ig (same clone as 66911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66911-1-Ig (MAVS; VISA antibody) at dilution of 1:1100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66911-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50509-3

  Cytometric bead array standard curve of MP50509-3, MAVS; VISA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66911-1-PBS. Detection antibody: 66911-4-PBS. Standard:Ag5949. Range: 0.391-50 ng/mL.

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Product Information

66911-1-PBS targets MAVS; VISA as part of a matched antibody pair:

MP50509-3: 66911-1-PBS capture and 66911-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAVS; VISA fusion protein Ag5949
• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-55 kDa, 70-75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.