MCP1 Antibody
MCP1 Polyclonal Antibody for FC, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 25542-1-AP
Synonyms
C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 6 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
The 25542-1-AP immunogen is Fusion Protein MCP1 protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCP1 fusion protein Ag10601 |
| Full Name | chemokine (C-C motif) ligand 2 |
| Calculated molecular weight | 99aa,11 kDa |
| Observed molecular weight | |
| GenBank accession number | BC009716 |
| Gene symbol | CCL2 |
| Gene ID (NCBI) | 6347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MCP1 antibody 25542-1-AP | Download protocol |
| IF protocol for MCP1 antibody 25542-1-AP | Download protocol |
| FC protocol for MCP1 antibody 25542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma. | ||
J Am Heart Assoc Toll-Like Receptor 9 Plays a Pivotal Role in Angiotensin II-Induced Atherosclerosis. | ||
Toxicology Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. | ||
Transl Res Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice. | ||
J Exp Clin Cancer Res PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway. | ||
J Cell Mol Med Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. |
