MCP1 Polyclonal antibody
MCP1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF, FC, Blocking, ELISA
Conjugate
Unconjugated
Cat no : 25542-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 14 publications below |
| IHC | See 15 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 25542-1-AP is MCP1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCP1 fusion protein Ag10601 |
| Full Name | chemokine (C-C motif) ligand 2 |
| Calculated molecular weight | 99 aa, 11 kDa |
| Observed molecular weight | 11 kDa |
| GenBank accession number | BC009716 |
| Gene symbol | CCL2 |
| Gene ID (NCBI) | 6347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCP1 antibody 25542-1-AP | Download protocol |
| IHC protocol for MCP1 antibody 25542-1-AP | Download protocol |
| IF protocol for MCP1 antibody 25542-1-AP | Download protocol |
| FC protocol for MCP1 antibody 25542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma. | ||
J Am Heart Assoc Toll-Like Receptor 9 Plays a Pivotal Role in Angiotensin II-Induced Atherosclerosis. | ||
Toxicology Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. | ||
Transl Res Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice. | ||
J Exp Clin Cancer Res PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway. | ||
J Cell Mol Med Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. |
