Validation Data Gallery
|Positive WB detected in||PMA, LPS and Brefeldin A treated THP-1 cells|
|Positive IHC detected in||human breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||A549 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25542-1-AP is MCP1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MCP1 fusion protein Ag10601|
|Full Name||chemokine (C-C motif) ligand 2|
|Calculated molecular weight||99 aa, 11 kDa|
|Observed molecular weight||11 kDa|
|GenBank accession number||BC009716|
|Gene ID (NCBI)||6347|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
|Product Specific Protocols|
|WB protocol for MCP1 antibody 25542-1-AP||Download protocol|
|IHC protocol for MCP1 antibody 25542-1-AP||Download protocol|
|IF protocol for MCP1 antibody 25542-1-AP||Download protocol|
|FC protocol for MCP1 antibody 25542-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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