MCP-1/CCL2 Polyclonal antibody

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  WB analysis using 25542-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 25542-1-AP (MCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25542-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MCP1 Polyclonal Antibody for IHC, ELISA

Cat No. 25542-1-AP

Publications(67)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, ELISA, Blocking

Synonyms

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

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Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells
• Positive IHC detected in: human breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 29 publications below
• IF: See 12 publications below

Product Information

25542-1-AP targets MCP1 in IHC, IF, ELISA, Blocking applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCP1 fusion protein Ag10601
• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC009716
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• RRID: AB_2880126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13500
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
human	IHC	Bone Res; Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome; Authors - Yanqiu Wang
human		J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
human	IHC,IF	Cancer Lett; LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer; Authors - Hansen Lin
	IF	J Hazard Mater; Polystyrene microplastics induced male reproductive toxicity in mice.; Authors - Haibo Jin
rabbit	IHC	Theranostics; Macrophage targeted theranostic strategy for accurate detection and rapid stabilization of the inflamed high-risk plaque.; Authors - Joon Woo Song