MCP1 Polyclonal antibody

MCP1 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)


IF, FC, IHC, ELISA, Blocking



Cat no : 25542-1-AP


C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPMA treated THP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25542-1-AP targets MCP1 in IF, FC, IHC, ELISA, Blocking applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCP1 fusion protein Ag10601
Full Name chemokine (C-C motif) ligand 2
Calculated Molecular Weight 99 aa, 11 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC009716
Gene Symbol CCL2
Gene ID (NCBI) 6347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.


Product Specific Protocols
WB protocol for MCP1 antibody 25542-1-APDownload protocol
IHC protocol for MCP1 antibody 25542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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