MCP1 Polyclonal antibody
MCP1 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, FC, Blocking, ELISA
Conjugate
Unconjugated
Cat no : 25542-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | PMA treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 24 publications below |
| IF | See 7 publications below |
Product Information
25542-1-AP targets MCP1 in WB, IHC, IF, FC, Blocking, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCP1 fusion protein Ag10601 |
| Full Name | chemokine (C-C motif) ligand 2 |
| Calculated Molecular Weight | 99 aa, 11 kDa |
| Observed Molecular Weight | 11 kDa |
| GenBank Accession Number | BC009716 |
| Gene Symbol | CCL2 |
| Gene ID (NCBI) | 6347 |
| RRID | AB_2880126 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCP1 antibody 25542-1-AP | Download protocol |
| IHC protocol for MCP1 antibody 25542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
J Neurol Neurosurg Psychiatry Molecules of senescent glial cells differentiate Alzheimer's disease from ageing | ||
Theranostics Macrophage targeted theranostic strategy for accurate detection and rapid stabilization of the inflamed high-risk plaque. | ||
Theranostics Reversing activity of cancer associated fibroblast for staged glycolipid micelles against internal breast tumor cells. | ||
Int J Cancer Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma. |
