MCP1 Polyclonal antibody

MCP1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, Blocking, ELISA

Conjugate

Unconjugated

Cat no : 25542-1-AP

Synonyms

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25542-1-AP is MCP1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCP1 fusion protein Ag10601
Full Name chemokine (C-C motif) ligand 2
Calculated molecular weight 99 aa, 11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC009716
Gene symbol CCL2
Gene ID (NCBI) 6347
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MCP1 antibody 25542-1-APDownload protocol
IHC protocol for MCP1 antibody 25542-1-APDownload protocol
IF protocol for MCP1 antibody 25542-1-APDownload protocol
FC protocol for MCP1 antibody 25542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Int J Cancer

Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma.

Authors - Qun Chen

J Am Heart Assoc

Toll-Like Receptor 9 Plays a Pivotal Role in Angiotensin II-Induced Atherosclerosis.

Authors - Daiju Fukuda
mouseWB

Toxicology

Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway.

Authors - Ruiyu Wang
mouseWB

Transl Res

Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice.

Authors - Chin-Chuan Chen
humanIHC

J Exp Clin Cancer Res

PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway.

Authors - Hui Shen
mouseWB

J Cell Mol Med

Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways.

Authors - Cheng Zheng