MCP1 Polyclonal antibody

Name: MCP1 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 25542-1-AP

MCP1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, FC, Blocking, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25542-1-AP

Synonyms

C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

Validation Data Gallery

WB analysis using 25542-1-AP
Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 25542-1-AP (MCP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 25542-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human breast cancer using 25542-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP (MCP1 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 25542-1-AP
Immunofluorescent analysis of HeLa cells using 25542-1-AP (MCP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of A549 using 25542-1-AP
1X10^6 A549 cells were stained with 0.2ug MCP1 antibody (25542-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Cat No. 25542-1-AP

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Tested Applications

Positive WB detected in	PMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected in	human breast cancer tissue, human pancreas cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells
Positive FC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 13 publications below
IHC	See 13 publications below
IF	See 3 publications below

Product Information

The immunogen of 25542-1-AP is MCP1 Fusion Protein expressed in E. coli.

Tested Reactivity	human
Cited Reactivity	human, mouse, rabbit, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCP1 fusion protein Ag10601
Full Name	chemokine (C-C motif) ligand 2
Calculated molecular weight	99 aa, 11 kDa
Observed molecular weight	11 kDa
GenBank accession number	BC009716
Gene symbol	CCL2
Gene ID (NCBI)	6347
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MCP1 antibody 25542-1-AP	Download protocol
IHC protocol for MCP1 antibody 25542-1-AP	Download protocol
IF protocol for MCP1 antibody 25542-1-AP	Download protocol
FC protocol for MCP1 antibody 25542-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Int J Cancer Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma. Authors - Qun Chen View Article
		J Am Heart Assoc Toll-Like Receptor 9 Plays a Pivotal Role in Angiotensin II-Induced Atherosclerosis. Authors - Daiju Fukuda View Article
mouse	WB	Toxicology Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Authors - Ruiyu Wang View Article
mouse	WB	Transl Res Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice. Authors - Chin-Chuan Chen View Article
human	IHC	J Exp Clin Cancer Res PLEK2 promotes gallbladder cancer invasion and metastasis through EGFR/CCL2 pathway. Authors - Hui Shen View Article
mouse	WB	J Cell Mol Med Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. Authors - Cheng Zheng View Article