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MCP1 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 25542-1-AP

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Con: 43 μg/150 μl

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Tested Applications

Applications:
IHC, IF, FC, ELISA
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Images See all

MCP1 Antibody IHC human breast cancer tissue 25542-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP( MCP1 antibody) at dilution of 1:200 (under 10x lens).

MCP1 Antibody IHC human breast cancer tissue 25542-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25542-1-AP( MCP1 antibody) at dilution of 1:200 (under 40x lens).

MCP1 Antibody IF HeLa cells 25542-1-AP

Immunofluorescent analysis of HeLa cells using 25542-1-AP(MCP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

MCP1 Antibody FC A549 cells 25542-1-AP

1X10^6 A549 cells were stained with 0.2ug MCP1 antibody (25542-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive IHC detected in:
human breast cancer tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
A549 cells
Recommended dilution:
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MCP1 fusion protein Ag10601
Full name:
chemokine (C-C motif) ligand 2
Calculated molecular weight:
99aa,11 kDa
Observed molecular weight:
GenBank accession number:
BC009716
Gene ID (NCBI):
6347
Gene symbol
CCL2
Synonyms
C C motif chemokine 2, CCL2, chemokine (C C motif) ligand 2, GDCF 2, HC11, HSMCR30, MCAF, MCP 1, MCP1, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2, SMC CF

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