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MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 10056-1-AP

Synonyms

MEF2C, myocyte enhancer factor 2C



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse colon tissue, mouse brain tissue, mouse heart tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10056-1-AP targets MEF2C in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag0020
Full Name myocyte enhancer factor 2C
Calculated Molecular Weight 51 kDa
Observed Molecular Weight45-70 kDa
GenBank Accession NumberBC156603
Gene Symbol MEF2C
Gene ID (NCBI) 4208
RRIDAB_513447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 10056-1-APDownload protocol
IHC protocol for MEF2C antibody 10056-1-APDownload protocol
IF protocol for MEF2C antibody 10056-1-APDownload protocol
IP protocol for MEF2C antibody 10056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Authors - Drukker Micha M
human,mouseIF

Cell Res

Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.

Authors - Xin-Xin Yu
humanWB

Nat Neurosci

Stimulus-specific combinatorial functionality of neuronal c-fos enhancers.

Authors - Jae-Yeol Joo
mouseIHC

Nat Neurosci

Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality.

Authors - Tzyy-Nan Huang
mouseWB

Nat Commun

NitroSynapsin therapy for a mouse MEF2C haploinsufficiency model of human autism.

Authors - Shichun Tu
mouseIHC

Genes Dev

Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.

Authors - López-Juárez Alejandro A