• Featured Product
  • KD/KO Validated

MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 10056-1-AP

Synonyms

MEF2C, myocyte enhancer factor 2C



Tested Applications

Positive WB detected inSH-SY5Y cells, mouse colon tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10056-1-AP targets MEF2C in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEF2C fusion protein Ag0020
Full Name myocyte enhancer factor 2C
Calculated molecular weight 51 kDa
Observed molecular weight 52 kDa, 60 kDa
GenBank accession numberBC156603
Gene symbol MEF2C
Gene ID (NCBI) 4208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 10056-1-APDownload protocol
IHC protocol for MEF2C antibody 10056-1-APDownload protocol
IF protocol for MEF2C antibody 10056-1-APDownload protocol
IP protocol for MEF2C antibody 10056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Med

Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.

Authors - Mingli Yin
mouseWB

PLoS One

Placental P-glycoprotein inhibition enhances susceptibility to Di-(2-ethylhexyl)-phthalate induced cardiac malformations in mice: A possibly promising target for congenital heart defects prevention.

Authors - Changqing Tang
mouseWB

J Appl Toxicol

The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.

Authors - Changqing Tang
humanWB,IF

Toxicol Appl Pharmacol

Novel formononetin-7-sal ester ameliorates pulmonary fibrosis via MEF2c signaling pathway.

Authors - Xueying Zhao
  • KD Validated
mouseIF

Cell Rep

Interhemispheric Connectivity Potentiates the Basolateral Amygdalae and Regulates Social Interaction and Memory.

Authors - Tzyy-Nan Huang
mouseIHC

Neural Dev

Septal contributions to olfactory bulb interneuron diversity in the embryonic mouse telencephalon: role of the homeobox gene Gsx2.

Authors - Shenyue Qin