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c-Met (Cytoplasmic) Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 25869-1-AP

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Con: 43 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

c-Met (Cytoplasmic) Antibody WB HepG2 cells 25869-1-AP

WB result of c-Met antibody (25869-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met transfected HepG2 cells

c-Met (Cytoplasmic) Antibody WB A431 cells 25869-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP( c-Met (Cytoplasmic) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours

c-Met (Cytoplasmic) Antibody IP HeLa cells 25869-1-AP

IP Result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

c-Met (Cytoplasmic) Antibody IHC human breast cancer tissue 25869-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25869-1-AP( c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens)

c-Met (Cytoplasmic) Antibody IHC human breast cancer tissue 25869-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25869-1-AP( c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, rat
Positive WB detected in:
A431 cells, HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue, human colon tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
c-Met (Cytoplasmic) fusion protein Ag23140
Full name:
met proto-oncogene (hepatocyte growth factor receptor)
Calculated molecular weight:
1390aa,155 kDa
Observed molecular weight:
145 kDa
GenBank accession number:
BC130420
Gene ID (NCBI):
4233
Gene symbol
MET
Synonyms
AUTS9, c-Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

Background

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.


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