c-Met (Cytoplasmic) Antibody

• Featured Product
• KD/KO Validated

c-Met (Cytoplasmic) Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 25869-1-AP

Synonyms

AUTS9, c-Met, HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

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Validation Data Gallery

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  WB analysis of HepG2 cells using 25869-1-AP

  WB result of c-Met antibody (25869-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met transfected HepG2 cells.

• 

  WB analysis of A431 cells using 25869-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 25869-1-AP

  IP Result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human breast cancer tissue using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer tissue using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 25869-1-AP is c-Met (Cytoplasmic) Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Met (Cytoplasmic) fusion protein Ag23140
• Full Name: met proto-oncogene (hepatocyte growth factor receptor)
• Calculated molecular weight: 1390 aa, 155 kDa
• Observed molecular weight: 145 kDa
• GenBank accession number: BC130420
• Gene symbol: MET
• Gene ID (NCBI): 4233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Publications

Species	Application	Title
human	IP	Autophagy; The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.; Authors - Xing Huang ; KO Validated
human	WB	Front Oncol; SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.; Authors - Ruoyan Liu
	WB	Oxid Med Cell Longev; PMID: 31781338; Authors - Ya-Dong Liu
human	WB	Kaohsiung J Med Sci; Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.; Authors - Jia-Jie Chen
human	WB	Thorac Cancer; CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.; Authors - Yichuan Chen
human	WB	Invest New Drugs; Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors.; Authors - Xingxing Yuan

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