c-Met (Cytoplasmic) Polyclonal antibody

• Featured Product
• KD/KO Validated

c-Met (Cytoplasmic) Polyclonal Antibody for FC (intra), IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine, mouse, rat

Applications

WB, IP, IHC, IF, FC (intra), ELISA

Conjugate

Unconjugated

Publications

38

Cat no : 25869-1-AP

Synonyms

AUTS9, c-Met, c-Met (Cytoplasmic), HGF receptor, HGF/SF receptor, HGFR, MET, Proto oncogene c Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine protein kinase Met

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Validation Data Gallery

• 

  WB analysis using 25869-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25869-1-AP

  WB result of c-Met antibody (25869-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met transfected HepG2 cells.

• 

  WB analysis of MDCK using 25869-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25869-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25869-1-AP

  IP Result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

• 

  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 25869-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-Met (Cytoplasmic) (25869-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse liver tissue, MDCK cells, HepG2 cells, A431 cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 29 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

25869-1-AP targets c-Met (Cytoplasmic) in WB, IP, IHC, IF, FC (intra), ELISA applications and shows reactivity with human, canine, mouse, rat samples.

• Tested Reactivity: human, canine, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Met (Cytoplasmic) fusion protein Ag23140
• Full Name: met proto-oncogene (hepatocyte growth factor receptor)
• Calculated molecular weight: 1390 aa, 155 kDa
• Observed molecular weight: 145 kDa
• GenBank accession number: BC130420
• Gene symbol: MET
• Gene ID (NCBI): 4233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Met (also named as MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis and survival. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, and esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Publications

Species	Application	Title
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
rat	WB	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui
human	WB	Int J Mol Sci; Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling; Authors - Xinglian Xu
human	WB	Mol Ther Nucleic Acids; Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.; Authors - Yalei Cui
human	IHC	Front Oncol; A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma; Authors - Hao Wang

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