c-Met (Cytoplasmic) Polyclonal antibody

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  WB analysis using 25869-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25869-1-AP

  WB result of c-Met (Cytoplasmic) antibody (25869-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-c-Met (Cytoplasmic) transfected HepG2 cells.

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  WB analysis of MDCK using 25869-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25869-1-AP

  IP result of anti-c-Met (Cytoplasmic) (IP:25869-1-AP, 5ug; Detection:25869-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25869-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25869-1-AP (c-Met (Cytoplasmic) antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 25869-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using c-Met (Cytoplasmic) antibody (25869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 25869-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human c-Met (Cytoplasmic) (25869-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

c-Met (Cytoplasmic) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 25869-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

c-Met, MET, HGF/SF receptor, HGF receptor, EC:2.7.10.1

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, MDCK cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 40 publications below
• IHC: See 13 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

25869-1-AP targets c-Met (Cytoplasmic) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Met (Cytoplasmic) fusion protein Ag23140
• Full Name: met proto-oncogene (hepatocyte growth factor receptor)
• Calculated Molecular Weight: 1390 aa, 155 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC130420
• Gene Symbol: MET
• Gene ID (NCBI): 4233
• RRID: AB_2880276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08581
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Met (also named MET or HGFR) is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to the HGF ligand. c-Met regulates many physiological processes including proliferation, scattering, morphogenesis, and survival. The primary single-chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide-linked to form the mature receptor. Overexpression and/or mutation of c-Met has been reported in various human malignancies, including lung cancer, breast cancer, head and neck cancer, gastric cancer, colorectal cancer, bladder cancer, uterine cervix carcinoma, esophageal carcinoma, c-Met could serve as an important therapeutic target (PMID: 26036285). The c-met receptor is a 190-kD glycoprotein consisting of a 145-kD membrane-spanning beta chain and a 50-kD alpha chain (PMID: 7806559). In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Metab; LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration; Authors - Yalan Deng
rat	WB	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
	WB	Oxid Med Cell Longev; PMID: 31781338; Authors - Ya-Dong Liu
human	IHC	Mol Ther Nucleic Acids; miRNA in food simultaneously controls animal viral disease and human tumorigenesis.; Authors - Yalei Cui
human	WB	Mol Ther Nucleic Acids; Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.; Authors - Yalei Cui