MFF Polyclonal antibody

• Featured Product
• KD/KO Validated

MFF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

121

Reviews (2)

Cat no : 17090-1-AP

Synonyms

C2orf33, MFF, mitochondrial fission factor

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Validation Data Gallery

• 

  WB analysis using 17090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17090-1-AP (MFF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17090-1-AP (MFF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17090-1-AP

  IP Result of anti-MFF (IP:17090-1-AP, 3ug; Detection:17090-1-AP 1:1000) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFF antibody (17090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 12 publications below
• WB: See 109 publications below
• IHC: See 4 publications below
• IF: See 18 publications below
• IP: See 3 publications below

Product Information

17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Chinese hamster ovary (CHO) cell, hamster, monkey, squirrel
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFF fusion protein Ag9990
• Full Name: mitochondrial fission factor
• Calculated molecular weight: 38 kDa
• Observed molecular weight: 35-38 kDa
• GenBank accession number: BC000797
• Gene symbol: MFF
• Gene ID (NCBI): 56947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publications

Species	Application	Title
human	WB	Front Genet; Clinical and Biochemical Features in a Patient With Mitochondrial Fission Factor Gene Alteration.; Authors - Alessia Nasca
human	WB	Proc Natl Acad Sci U S A; Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.; Authors - Chih-Wei Chen
mouse	WB	J Neurosci; Acquired expression of mutant Mitofusin 2 causes progressive neurodegeneration and abnormal behavior.; Authors - Kaori Ishikawa
rat	IF	Cell; Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity.; Authors - Vidhya Rangaraju
squirrel	WB	J Cell Physiol; Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.; Authors - Zhe Wang
mouse	WB	Mol Cell; The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.; Authors - Davide D'Amico

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 and HeLa

FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP

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