MFF Polyclonal antibody

• Featured Product
• KD/KO Validated

MFF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

180

Reviews (2)

Cat no : 17090-1-AP

Synonyms

C2orf33, MFF, mitochondrial fission factor

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Validation Data Gallery

• 

  WB analysis using 17090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17090-1-AP (MFF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17090-1-AP

  IP result of anti-MFF (IP:17090-1-AP, 4ug; Detection:17090-1-AP 1:30000) with mouse brain tissue lysate 1680 ug.

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human stomach using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17090-1-AP (MFF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFF antibody (17090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 163 publications below
• IHC: See 6 publications below
• IF: See 24 publications below
• IP: See 4 publications below

Product Information

17090-1-AP targets MFF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, hamster, goat, squirrel
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFF fusion protein Ag9990
• Full Name: mitochondrial fission factor
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC000797
• Gene Symbol: MFF
• Gene ID (NCBI): 56947
• RRID: AB_2142463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Publications

Species	Application	Title
human	WB	Nature; Multiple dynamin family members collaborate to drive mitochondrial division.; Authors - Jason E Lee
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Nature; Correcting mitochondrial fusion by manipulating mitofusin conformations.; Authors - Antonietta Franco
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
monkey	WB	Science; ER tubules mark sites of mitochondrial division.; Authors - Jonathan R Friedman ; KD Validated
human	WB	Cell; CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.; Authors - Philipp Hammerschmidt

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293 and HeLa

FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP

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