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  • KD/KO Validated

MFF Polyclonal antibody

MFF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17090-1-AP

Synonyms

C2orf33, MFF, mitochondrial fission factor



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Chinese hamster ovary (CHO) cell, hamster, monkey, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFF fusion protein Ag9990
Full Name mitochondrial fission factor
Calculated molecular weight 38 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC000797
Gene symbol MFF
Gene ID (NCBI) 56947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Protocols

Product Specific Protocols
WB protocol for MFF antibody 17090-1-APDownload protocol
IHC protocol for MFF antibody 17090-1-APDownload protocol
IF protocol for MFF antibody 17090-1-APDownload protocol
IP protocol for MFF antibody 17090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Multiple dynamin family members collaborate to drive mitochondrial division.

Authors - Jason E Lee
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
mouseWB

Nature

Correcting mitochondrial fusion by manipulating mitofusin conformations.

Authors - Antonietta Franco
monkeyWB

Science

ER tubules mark sites of mitochondrial division.

Authors - Jonathan R Friedman
  • KD Validated
humanWB

Cell

CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.

Authors - Philipp Hammerschmidt
humanWB

Nat Cell Biol

Molecular basis of selective mitochondrial fusion by heterotypic action between OPA1 and cardiolipin.

Authors - Tadato Ban

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (09-07-2020)

This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HEK293 and HeLa
FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP