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MFF Antibody

MFF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17090-1-AP

Synonyms

C2orf33, MFF, mitochondrial fission factor



Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human heart tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat, squirrels
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFF fusion protein Ag9990
Full Name mitochondrial fission factor
Calculated molecular weight 38 kDa
Observed molecular weight 26-29, 35-38 kDa
GenBank accession number BC000797
Gene symbol MFF
Gene ID (NCBI) 56947
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.

Protocols

Product Specific Protocols
WB protocol for MFF antibody 17090-1-APDownload protocol
IHC protocol for MFF antibody 17090-1-APDownload protocol
IF protocol for MFF antibody 17090-1-APDownload protocol
IP protocol for MFF antibody 17090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

Clinical and Biochemical Features in a Patient With Mitochondrial Fission Factor Gene Alteration.

Authors - Alessia Nasca
humanWB

Proc Natl Acad Sci U S A

Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder.

Authors - Chih-Wei Chen
mouseWB

J Neurosci

Acquired expression of mutant Mitofusin 2 causes progressive neurodegeneration and abnormal behavior.

Authors - Kaori Ishikawa
ratIF

Cell

Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity.

Authors - Vidhya Rangaraju
squirrelsWB

J Cell Physiol

Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.

Authors - Zhe Wang
mouseWB

Mol Cell

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.

Authors - Davide D'Amico

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

JAE HO (Verified Customer) (04-22-2019)

I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: PC3/MDAMB231/LN229/MCF7/MCF10/DU145/LnCaP
No image submitted with review