Validation Data Gallery
|Positive WB detected in||mouse brain tissue, rat brain tissue, mouse heart tissue, rat heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human stomach tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Chinese hamster ovary (CHO) cell, hamster, monkey, squirrel|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MFF fusion protein Ag9990|
|Full Name||mitochondrial fission factor|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC000797|
|Gene ID (NCBI)||56947|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
|Product Specific Protocols|
|WB protocol for MFF antibody 17090-1-AP||Download protocol|
|IHC protocol for MFF antibody 17090-1-AP||Download protocol|
|IF protocol for MFF antibody 17090-1-AP||Download protocol|
|IP protocol for MFF antibody 17090-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Multiple dynamin family members collaborate to drive mitochondrial division.
Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.
Correcting mitochondrial fusion by manipulating mitofusin conformations.
ER tubules mark sites of mitochondrial division.
CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity.
Nat Cell Biol
Molecular basis of selective mitochondrial fusion by heterotypic action between OPA1 and cardiolipin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chun (Verified Customer) (09-07-2020)
This antibody is good for IP, and there are non-specific band(s) detected when it is used for immunoblotting.
JAE HO (Verified Customer) (04-22-2019)
I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.