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MFF Antibody
MFF Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17090-1-AP
Synonyms
C2orf33, MFF, mitochondrial fission factor
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human heart tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 55 publications below |
| IHC | See 1 publications below |
| IF | See 11 publications below |
| IP | See 2 publications below |
Product Information
17090-1-AP targets MFF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey, mouse, rat, squirrels |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MFF fusion protein Ag9990 |
| Full Name | mitochondrial fission factor |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 26-29, 35-38 kDa |
| GenBank accession number | BC000797 |
| Gene symbol | MFF |
| Gene ID (NCBI) | 56947 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MFF antibody 17090-1-AP | Download protocol |
| IHC protocol for MFF antibody 17090-1-AP | Download protocol |
| IF protocol for MFF antibody 17090-1-AP | Download protocol |
| IP protocol for MFF antibody 17090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Genet Clinical and Biochemical Features in a Patient With Mitochondrial Fission Factor Gene Alteration. | ||
Proc Natl Acad Sci U S A Two separate functions of NME3 critical for cell survival underlie a neurodegenerative disorder. | ||
J Neurosci Acquired expression of mutant Mitofusin 2 causes progressive neurodegeneration and abnormal behavior. | ||
Cell Spatially Stable Mitochondrial Compartments Fuel Local Translation during Plasticity. | ||
J Cell Physiol Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen. | ||
Mol Cell The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH JAE HO (Verified Customer) (04-22-2019) | I used couple of antibodies for MFF proteins. Only this antibody was working nicely. I satisfied its higher titer and specificity. I have tested and used this antibody for WB and IP.
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