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MFF Antibody 48 Publications

Rabbit Polyclonal| Catalog number: 17090-1-AP

Featured Product KD/KO validated

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

MFF Antibody IP mouse brain tissue 17090-1-AP

IP Result of anti-MFF (IP:17090-1-AP, 3ug; Detection:17090-1-AP 1:1000) with mouse brain tissue lysate 8000ug.

MFF Antibody IHC human brain tissue 17090-1-AP

Immunohistochemical of paraffin-embedded human brain using 17090-1-AP(MFF antibody) at dilution of 1:100 (under 10x lens)

MFF Antibody IHC human brain tissue 17090-1-AP

Immunohistochemical of paraffin-embedded human brain using 17090-1-AP(MFF antibody) at dilution of 1:100 (under 40x lens)

MFF Antibody WB human brain tissue 17090-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 17090-1-AP(MFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MFF Antibody IF HepG2 cells 17090-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17090-1-AP(MFF antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, monkey, squirrels
Positive WB detected in:
human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human brain tissue, human heart tissue, human stomach tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, Hela cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MFF fusion protein Ag9990
Full name:
mitochondrial fission factor
Calculated molecular weight:
38 kDa
Observed molecular weight:
26-29, 35-38 kDa
GenBank accession number:
BC000797
Gene ID (NCBI):
56947
Gene symbol
MFF
Synonyms
C2orf33, MFF, mitochondrial fission factor

Background

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.


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