Validation Data Gallery
|Positive WB detected in||HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, U-251 cells, U-87 MG cells|
|Positive IHC detected in||human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66776-1-Ig targets MFN1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||MFN1 fusion protein Ag4890|
|Full Name||mitofusin 1|
|Calculated molecular weight||741 aa, 84 kDa|
|Observed molecular weight||84 kDa|
|GenBank accession number||BC040557|
|Gene ID (NCBI)||55669|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects.
Ecotoxicol Environ Saf
Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis
Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.
J Mol Histol
Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats
Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis
J Nutr Biochem
miR-494-5p mediates the antioxidant activity of EPA by targeting the mitochondrial elongation factor 1 gene MIEF1 in HepG2 cells
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
benjamin (Verified Customer) (01-31-2019)
Very strong for WB with 20ug of protein using heLa cells