MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

17

CloneNo.

3F11C11

Reviews (1)

Cat no : 66776-1-Ig

Synonyms

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

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Validation Data Gallery

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  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66776-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66776-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66776-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, U-251 cells, U-87 MG cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

66776-1-Ig targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MFN1 fusion protein Ag4890
• Full Name: mitofusin 1
• Calculated Molecular Weight: 741 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC040557
• Gene Symbol: MFN1
• Gene ID (NCBI): 55669
• RRID: AB_2882122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Publications

Species	Application	Title
human	IF	Nat Commun; BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects.; Authors - Gee Euhn Choi
pig	IF	Ecotoxicol Environ Saf; Zinc deficiency compromises the maturational competence of porcine oocyte by inducing mitophagy and apoptosis; Authors - Xin-Le Lai
human	WB	Molecules; Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.; Authors - Jiling Feng
rat	IHC	J Mol Histol; Roles of HIF-1α/BNIP3 mediated mitophagy in mitochondrial dysfunction of letrozole-induced PCOS rats; Authors - Fan Wang
human,rat	WB	Phytomedicine; Xiangsha Liujunzi Decoction improves gastrointestinal motility in functional dyspepsia with spleen deficiency syndrome by restoring mitochondrial quality control homeostasis; Authors - Jiaqi Zhang
human	WB	J Nutr Biochem; miR-494-5p mediates the antioxidant activity of EPA by targeting the mitochondrial elongation factor 1 gene MIEF1 in HepG2 cells; Authors - Yanqin Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

benjamin (Verified Customer) (01-31-2019)

Very strong for WB with 20ug of protein using heLa cells

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa PWPI