CoraLite®594-conjugated MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F11C11

Cat no : CL594-66776

Synonyms

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1



Tested Applications

Positive IF detected inHepG2 cells, T-47D cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66776-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66776 targets MFN1 in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MFN1 fusion protein Ag4890
Full Name mitofusin 1
Calculated molecular weight 741 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC040557
Gene symbol MFN1
Gene ID (NCBI) 55669
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Protocols

Product Specific Protocols
IF protocol for CL594 MFN1 antibody CL594-66776Download protocol
FC protocol for CL594 MFN1 antibody CL594-66776Download protocol
Standard Protocols
Click here to view our Standard Protocols