Validation Data Gallery
|Positive WB detected in||A549 cells, U-937 cells, HL-60 cells, Y79 cells, rat spleen tissue, HEK-293T cells, HUVEC cells, Jurkat cells, U-87 MG cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IF detected in||U-251 cells|
|Positive FC detected in||THP-1 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20415-1-AP targets MIF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MIF fusion protein Ag14058|
|Full Name||macrophage migration inhibitory factor (glycosylation-inhibiting factor)|
|Calculated molecular weight||115 aa, 12 kDa|
|Observed molecular weight||12 kDa|
|GenBank accession number||BC000447|
|Gene ID (NCBI)||4282|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.
Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.
TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).
Food Chem Toxicol
The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation
Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.
Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.
A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (01-24-2023)
This antibody worked well despite low protein concentration.
Emma (Verified Customer) (03-15-2022)
Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size.
Ryan (Verified Customer) (02-27-2019)
Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).