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MIF Polyclonal antibody
MIF Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 20415-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, U-937 cells, HL-60 cells, Y79 cells, rat spleen tissue, HEK-293T cells, HUVEC cells, Jurkat cells, U-87 MG cells |
Positive IP detected in | mouse spleen tissue |
Positive IF detected in | U-251 cells |
Positive FC detected in | THP-1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 5 publications below |
WB | See 9 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
Product Information
20415-1-AP targets MIF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MIF fusion protein Ag14058 |
Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
Calculated molecular weight | 115 aa, 12 kDa |
Observed molecular weight | 12 kDa |
GenBank accession number | BC000447 |
Gene symbol | MIF |
Gene ID (NCBI) | 4282 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.
Protocols
Product Specific Protocols | |
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WB protocol for MIF antibody 20415-1-AP | Download protocol |
IF protocol for MIF antibody 20415-1-AP | Download protocol |
IP protocol for MIF antibody 20415-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.
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Cell Prolif TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD). | ||
Food Chem Toxicol The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation | ||
Mol Carcinog Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.
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BMC Microbiol Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection. | ||
Mol Biosyst A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (01-24-2023) | This antibody worked well despite low protein concentration.
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FH Emma (Verified Customer) (03-15-2022) | Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size.
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FH Ryan (Verified Customer) (02-27-2019) | Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).
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