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  • KD/KO Validated

MIF Polyclonal antibody

MIF Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, rat, mouse





Cat no : 20415-1-AP


GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase

Tested Applications

Positive WB detected inA549 cells, U-937 cells, HL-60 cells, Y79 cells, rat spleen tissue, HEK-293T cells, HUVEC cells, Jurkat cells, U-87 MG cells
Positive IP detected inmouse spleen tissue
Positive IF detected inU-251 cells
Positive FC detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20415-1-AP targets MIF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIF fusion protein Ag14058
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Calculated Molecular Weight 115 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000447
Gene Symbol MIF
Gene ID (NCBI) 4282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.


Product Specific Protocols
WB protocol for MIF antibody 20415-1-APDownload protocol
IF protocol for MIF antibody 20415-1-APDownload protocol
IP protocol for MIF antibody 20415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Lett

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Authors - Rui-Min Liu
  • KD Validated

Cell Prolif

TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).

Authors - Xiaojun Zhang

Front Endocrinol (Lausanne)

Exercise prevents fatal stress-induced myocardial injury in obese mice

Authors - Yaoshan Dun

Food Chem Toxicol

The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation

Authors - Siwen Li

Mol Carcinog

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Authors - Sha-Sha Wang
  • KD Validated

BMC Microbiol

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

Authors - Shuxian Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, incubated with AF594 for 2 h at RT

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Ovarian tissue
MIF Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:20415-1-AP)

sarah (Verified Customer) (10-27-2023)

antibody worked great for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T, HCT116

Sarah (Verified Customer) (01-24-2023)

This antibody worked well despite low protein concentration.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116

Emma (Verified Customer) (03-15-2022)

Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf
MIF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:20415-1-AP)