• Featured Product
  • KD/KO Validated

MIF Polyclonal antibody

MIF Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20415-1-AP

Synonyms

GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase



Tested Applications

Positive WB detected inA549 cells, U-937 cells, Y79 cells, rat spleen tissue, HL-60 cells, U-87 MG cells, HEK-293T cells
Positive IP detected inmouse spleen tissue
Positive IF detected inU-251 cells
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20415-1-AP is MIF Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIF fusion protein Ag14058
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Calculated molecular weight 115 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC000447
Gene symbol MIF
Gene ID (NCBI) 4282
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MIF antibody 20415-1-APDownload protocol
IF protocol for MIF antibody 20415-1-APDownload protocol
IP protocol for MIF antibody 20415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncotarget

Macrophage migration inhibitory factor promotes vasculogenic mimicry formation induced by hypoxia via CXCR4/AKT/EMT pathway in human glioblastoma cells.

Authors - Xing Guo
humanIF

Cell Death Discov

Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI.

Authors - Tianlong Li
  • KO Validated
humanWB

Proteome Sci

Proteomic analysis of human periodontal ligament cells under hypoxia.

Authors - Qiwen Li

Mol Biosyst

A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.

Authors - Langdong Chen
humanWB

BMC Microbiol

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

Authors - Shuxian Li
humanWB,IHC

Mol Carcinog

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Authors - Sha-Sha Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (03-15-2022)

Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PC3
FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf
MIF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:20415-1-AP)