MLKL Polyclonal antibody

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  WB analysis using 21066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21066-1-AP (MLKL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21066-1-AP

  IP result of anti-MLKL (IP:21066-1-AP, 4ug; Detection:21066-1-AP 1:1000) with HepG2 cells lysate 1470 ug.

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  IHC staining of human liver cancer using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21066-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21066-1-AP (MLKL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21066-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (21066-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

MLKL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21066-1-AP

Publications(63)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Mixed lineage kinase domain-like protein, hMLKL

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Tested Applications

• Positive WB detected in: HT-29 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 56 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

21066-1-AP targets MLKL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLKL fusion protein Ag15188
• Full Name: mixed lineage kinase domain-like
• Calculated Molecular Weight: 471 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC028141
• Gene Symbol: MLKL
• Gene ID (NCBI): 197259
• RRID: AB_2878804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NB16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Publications

Species	Application	Title
human	WB	Cell Host Microbe; Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.; Authors - Alex Rosenberg ; KO Validated
human	WB	Cancer Lett; Modulation of YBX1-mediated PANoptosis inhibition by PPM1B and USP10 confers chemoresistance to oxaliplatin in gastric cancer; Authors - Chunlin Lin
	WB	Front Immunol; N-acetyl-L-cysteine alleviated the oxidative stress-induced inflammation and necroptosis caused by excessive NiCl2 in primary spleen lymphocytes; Authors - Xintong Zhang
	WB,IF	Cell Death Discov; MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury; Authors - Jian Xu
human	WB	Cancers (Basel); STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.; Authors - Zhen-Yuan Zheng
	WB	Am J Chin Med; Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.; Authors - Xiaxia Wu