• Featured Product
  • KD/KO Validated

MLKL Polyclonal antibody

MLKL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21066-1-AP

Synonyms

BC028141, MLKL



Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells, mMSCs cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21066-1-AP targets MLKL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLKL fusion protein Ag15188
Full Name mixed lineage kinase domain-like
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC028141
Gene symbol MLKL
Gene ID (NCBI) 197259
RRIDAB_2878804
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

Protocols

Product Specific Protocols
WB protocol for MLKL antibody 21066-1-APDownload protocol
IHC protocol for MLKL antibody 21066-1-APDownload protocol
IF protocol for MLKL antibody 21066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Host Microbe

Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.

Authors - Alex Rosenberg
  • KO Validated
WB

Front Immunol

N-acetyl-L-cysteine alleviated the oxidative stress-induced inflammation and necroptosis caused by excessive NiCl2 in primary spleen lymphocytes

Authors - Xintong Zhang
WB,IF

Cell Death Discov

MLKL deficiency attenuated hepatocyte oxidative DNA damage by activating mitophagy to suppress macrophage cGAS-STING signaling during liver ischemia and reperfusion injury

Authors - Jian Xu
humanWB

Cancers (Basel)

STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.

Authors - Zhen-Yuan Zheng
WB

Am J Chin Med

Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.

Authors - Xiaxia Wu
pigWB

Front Cell Dev Biol

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model.

Authors - Jianni Huang