Tested Applications
| Positive WB detected in | A375 cells, A549 cells, LNCaP cells, MCF-7 cells |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10338-1-AP targets MLPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0316 Product name: Recombinant human MLPH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-236 aa of BC001653 Sequence: SKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKA Predict reactive species |
| Full Name | melanophilin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66 kDa, 80 kDa |
| GenBank Accession Number | BC001653 |
| Gene Symbol | MLPH |
| Gene ID (NCBI) | 79083 |
| RRID | AB_2146104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BV36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MLPH antibody 10338-1-AP | Download protocol |
| IHC protocol for MLPH antibody 10338-1-AP | Download protocol |
| IP protocol for MLPH antibody 10338-1-AP | Download protocol |
| WB protocol for MLPH antibody 10338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Melanophilin (MLPH) antibody, catalog number 10338-1-AP, has 32 citations. These appear in journals including Theranostics, Oncogene, Cell Death Dis, J Allergy Clin Immunol, Br J Dermatol, J Invest Dermatol, Sci Rep, J Dermatol Sci, Pigment Cell Melanoma Res, Mol Genet Metab, Elife, and others. Research fields span melanosome transport, melanogenesis and skin pigmentation, Griscelli syndrome, and cancer (pancreatic, prostate, glioblastoma), with applications mainly in Western blot, IF, IP, and IHC.
| Species | Application | Title |
|---|---|---|
Theranostics A novel function of Prohibitin on melanosome transport in melanocytes.
| ||
Theranostics Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction | ||
Cell Death Dis Melanophilin-induced primary cilia promote pancreatic cancer metastasis
| ||
J Allergy Clin Immunol Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding. | ||
Part Fibre Toxicol Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies. | ||
Oncogene O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination |
Reviews
What customers say
The product received a single 5-star review. The reviewer used it for Western Blot and Immunohistochemistry on skin tissue and reported "very good signal." Overall, the feedback highlights strong performance and clear signal quality across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ali (Verified Customer) (08-08-2025) | VERY GOOD SIGNAL
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