|Positive WB detected in||A375 cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10338-1-AP targets Melanophilin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Melanophilin fusion protein Ag0316|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66 kDa, 80 kDa|
|GenBank accession number||BC001653|
|Gene ID (NCBI)||79083|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
|Product Specific Protocols|
|WB protocol for Melanophilin antibody 10338-1-AP||Download protocol|
|IHC protocol for Melanophilin antibody 10338-1-AP||Download protocol|
|IF protocol for Melanophilin antibody 10338-1-AP||Download protocol|
|IP protocol for Melanophilin antibody 10338-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of MicroRNA Targeting Mlph and Affecting Melanosome Transport.
Mol Genet Metab
A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.
Onco Targets Ther
MLPH Accelerates the Epithelial-Mesenchymal Transition in Prostate Cancer.
Differential gene expression in normal esophagus and Barrett's esophagus.
J Dermatol Sci
16-Kauren-2-beta-18,19-triol inhibits melanosome transport in melanocytes by down-regulation of melanophilin expression.
Regulation of Melanophilin (Mlph) gene expression by the glucocorticoid receptor (GR).