• Featured Product
  • KD/KO Validated

Melanophilin Polyclonal antibody

Melanophilin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10338-1-AP

Synonyms

Exophilin 3, melanophilin, MLPH, SLAC2 A, SLAC2A, Synaptotagmin like protein 2a



Tested Applications

Positive WB detected inA375 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10338-1-AP targets Melanophilin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Melanophilin fusion protein Ag0316
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 80 kDa
GenBank accession numberBC001653
Gene symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_2146104
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.

Protocols

Product Specific Protocols
WB protocol for Melanophilin antibody 10338-1-APDownload protocol
IHC protocol for Melanophilin antibody 10338-1-APDownload protocol
IF protocol for Melanophilin antibody 10338-1-APDownload protocol
IP protocol for Melanophilin antibody 10338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP

J Allergy Clin Immunol

Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.

Authors - Valentina Cetica
humanWB

Theranostics

A novel function of Prohibitin on melanosome transport in melanocytes.

Authors - Chan Song Jo
  • KD Validated
humanWB,IF,IP

Oncogene

O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination

Authors - Lei Xu
mouseWB

Elife

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

Authors - Kunli Zhao
mouseWB,IF

Part Fibre Toxicol

Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.

Authors - Min Mu
humanWB

J Invest Dermatol

Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.

Authors - Van Gele Mireille M