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Melanophilin Polyclonal antibody
Melanophilin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10338-1-AP
|Positive WB detected in
|Positive IP detected in
|Positive IHC detected in
|human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, MCF-7 cells
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10338-1-AP targets Melanophilin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|Melanophilin fusion protein Ag0316
|Calculated molecular weight
|Observed molecular weight
|66 kDa, 80 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
|Product Specific Protocols
|WB protocol for Melanophilin antibody 10338-1-AP
|IHC protocol for Melanophilin antibody 10338-1-AP
|IF protocol for Melanophilin antibody 10338-1-AP
|IP protocol for Melanophilin antibody 10338-1-AP
|Click here to view our Standard Protocols
J Allergy Clin Immunol
Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding.
A novel function of Prohibitin on melanosome transport in melanocytes.
O-GlcNAcylation of melanophilin enhances radiation resistance in glioblastoma via suppressing TRIM21 mediated ubiquitination
Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis
Part Fibre Toxicol
Coal dust exposure triggers heterogeneity of transcriptional profiles in mouse pneumoconiosis and Vitamin D remedies.
J Invest Dermatol
Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes.