MMS2 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 10689-1-AP (same clone as 10689-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 10689-1-AP (same clone as 10689-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10689-1-AP (same clone as 10689-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10689-1-AP (same clone as 10689-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10689-1-AP (same clone as 10689-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10689-1-AP (same clone as 10689-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10689-1-AP (same clone as 10689-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10689-1-AP (same clone as 10689-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 10689-1-AP (same clone as 10689-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10689-1-AP (same clone as 10689-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10689-1-AP (same clone as 10689-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 10689-1-AP (same clone as 10689-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10689-1-AP (same clone as 10689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10689-1-AP (MMS2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10689-1-AP (same clone as 10689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10689-1-AP (same clone as 10689-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10689-1-AP (same clone as 10689-1-PBS)

  Immunofluorescent analysis of HeLa cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10689-1-AP (same clone as 10689-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10689-1-PBS in a different storage buffer formulation.

MMS2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 10689-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UBE2V2, EDPF1, EDPF 1, EDAF-1, EDAF 1

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Product Information

10689-1-PBS targets MMS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMS2 fusion protein Ag1111
• Full Name: ubiquitin-conjugating enzyme E2 variant 2
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC007051
• Gene Symbol: MMS2
• Gene ID (NCBI): 7336
• RRID: AB_2211656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15819
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.