Moesin Polyclonal antibody

Moesin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 26053-1-AP

Synonyms

moesin, MSN

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Validation Data Gallery

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  WB analysis of Jurkat using 26053-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26053-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26053-1-AP (MSN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 26053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26053-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26053-1-AP (MSN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26053-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26053-1-AP (MSN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 26053-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.20ug Anti-Human MSN (26053-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Neuro-2a cells, C6 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26053-1-AP targets Moesin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Moesin fusion protein Ag23531
• Full Name: moesin
• Calculated Molecular Weight: 577 aa, 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC017293
• Gene Symbol: MSN
• Gene ID (NCBI): 4478
• RRID: AB_2880353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Caitlin (Verified Customer) (09-10-2019)

Good Antibody for western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Thyroid Cancer Cell Lines