|Positive WB detected in||mouse heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||mouse heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue, mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:600-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22280-1-AP targets MYH7-specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||myosin, heavy chain 7, cardiac muscle, beta|
|Calculated molecular weight||223 kDa|
|Observed molecular weight||200-220 kDa|
|GenBank accession number||NM_000257|
|Gene ID (NCBI)||4625|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)
|Product Specific Protocols|
|WB protocol for MYH7-specific antibody 22280-1-AP||Download protocol|
|IHC protocol for MYH7-specific antibody 22280-1-AP||Download protocol|
|IF protocol for MYH7-specific antibody 22280-1-AP||Download protocol|
|IP protocol for MYH7-specific antibody 22280-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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