MYH7-specific Polyclonal antibody

MYH7-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22280-1-AP

Synonyms

beta MHC, CMD1S, CMH1, MPD1, MYH7, MyHC beta, MyHC slow, MYHCB, Myosin 7, Myosin heavy chain 7, SPMD, SPMM



Tested Applications

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

22280-1-AP targets MYH7-specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin, heavy chain 7, cardiac muscle, beta
Calculated molecular weight 223 kDa
Observed molecular weight 200-220 kDa
GenBank accession numberNM_000257
Gene symbol MYH7
Gene ID (NCBI) 4625
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)

Protocols

Product Specific Protocols
WB protocol for MYH7-specific antibody 22280-1-APDownload protocol
IHC protocol for MYH7-specific antibody 22280-1-APDownload protocol
IF protocol for MYH7-specific antibody 22280-1-APDownload protocol
IP protocol for MYH7-specific antibody 22280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Histochem

Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.

Authors - Li Lixin
ratWB,IF

Med Sci Monit

Roles of Mitogen-Activating Protein Kinase Kinase Kinase Kinase-3 (MAP4K3) in Preterm Skeletal Muscle Satellite Cell Myogenesis and Mammalian Target of Rapamycin Complex 1 (mTORC1) Activation Regulation.

Authors - Chu-Yi Guo
humanIF

Int J Cosmet Sci

Comprehensive histological investigation of age-related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion.

Authors - T Gomi
ratWB,IHC

Mol Med Rep

Proteomics of acute heart failure in a rat post-myocardial infarction model.

Authors - Yichen Guo
ratWB

Biochem Biophys Res Commun

Platelet-derived growth factor regulates vascular smooth muscle phenotype via mammalian target of rapamycin complex 1.

Authors - Jung Min Ha

J Agric Food Chem

Excess imidacloprid exposure causes the heart tube malformation of chick embryos.

Authors - Lin-Rui Gao