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MYH9 Polyclonal antibody

MYH9 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 11128-1-AP

Synonyms

MYH9, Myosin 9, Myosin heavy chain 9, myosin IIa, Nonmuscle myosin IIa



Tested Applications

Positive WB detected inA549 cells, mouse colon tissue, mouse kidney tissue, mouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11128-1-AP targets MYH9 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYH9 fusion protein Ag1612
Full Name myosin, heavy chain 9, non-muscle
Calculated molecular weight 227 kDa
Observed molecular weight 224 kDa
GenBank accession numberBC011915
Gene symbol MYH9
Gene ID (NCBI) 4627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Protocols

Product Specific Protocols
WB protocol for MYH9 antibody 11128-1-APDownload protocol
IHC protocol for MYH9 antibody 11128-1-APDownload protocol
IF protocol for MYH9 antibody 11128-1-APDownload protocol
IP protocol for MYH9 antibody 11128-1-APDownload protocol
FC protocol for MYH9 antibody 11128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Invest Ophthalmol Vis Sci

SWATH-MS Proteomic Analysis of Oxygen-Induced Retinopathy Reveals Novel Potential Therapeutic Targets.

Authors - Maria Vähätupa
humanWB,CoIP

Cell Death Dis

NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Authors - Wenwen Chen
humanIHC

Oncol Lett

MYH9 overexpression correlates with clinicopathological parameters and poor prognosis of epithelial ovarian cancer.

Authors - Longyang Liu
humanIHC

Reproduction

Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.

Authors - Ru Zheng
humanWB,IP,IF

Exp Cell Res

The Actomyosin Network is Influenced by NMHC IIA and Regulated by CrpF46, which is Involved in Controlling Cell Migration.

Authors - Yang Cao
humanWB

Am J Pathol

S100A4/Nonmuscle Myosin IIA/p53 Axis Contributes to Aggressive Features in Ovarian High-Grade Serous Carcinoma.

Authors - Ai Hiruta